Barramundi (Lates calcarifer) fish is one of the fishery commodities in Indonesia which has economic value and is the most exported commodity. The problem that often occurs in the process of cultivating barramundi (Lates calcarifer) is a parasite from the Haliotrema sp. which attacks the gills and can cause a decrease in the level of fish production. One way to determine the parasite infesting barramundi (Lates calcarifer) is by conducting a molecular identification. This study aims to determine the parasites by molecular identification and prevalence rate of ectoparasite worms in the gills of barramundi (Lates calcalifer) fish in Lampung waters. A sample of 40 fish with a length of 25-35 cm was taken by using a purposive sampling method. The organs observed were fish gills, then molecular identification was carried out by using real-time PCR, and the prevalence calculations. The results of this study indicated that the fish gills were infested by ectoparasitic worms, according to the NCBI GenBank database, called Haliotrema susanae with the band at 748 bp, and the prevalence rate of infested fish with gill’s ectoparasites is 80%.

Silver pompano (Trachinotus blochii) is one type of mari-culture that has high economic value and both national and international markets. The cultivation technique of silver pompano (T. blochii) used in floating net cages is inseparable from the common problems in every cultivation activity. one of these obstacles is the problem of infectious diseases, where is caused by disturbances this disease causes in fish metabolism by organisms such as parasites. The aim of this study was to identify the types of endoparasite worms based on molecular and the prevalence of endoparasite worms in silver pompano in floating net cages of Mari-culture Center, Lampung. It was done by taking 45 samples of fish with a length of 15-25 cm using a purposive sampling method. The observed internal organ of fish, then molecular identification, was carried out with conventional PCR and prevalence calculation. This study showed there was Camallanus carangid by DNA amplification fragments with a band of 972 bp and the prevalence value of 8.8%.