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the accumulation of the DETC-Fe-NO complex was completely blocked by 2 mM ethylene glycol tetraacetate, a specific chelator of Ca 2+ ions, which is an obligatory component of the NO-synthase reaction; 3) the accumulation of the DETC complex was inhibited by the specific inhibitors of NO-synthase N-methyl-L-arginine (by 70% at a concentration of 1 raM) and nitro-L-arginine (by 60% at a concentration of 1 raM).Thus, the method proposed here may be used for correct determination of NO-synthase activity in dense brain homogenates. The method is also applicable for enzyme preparations of various purity (in these cases auxiliary problems concerning the incubation conditions and addition of Fe(II) should be experimentally solved), but in the case of dense homogenates and tissue samples, where the level of effectors regulating NO-synthase activity is maximally preserved, this method offers the greatest advantages. REFERENCES I. P.A. Bush, N. E. Gortzales, J. M. Griscavage, and L. J.Ignarro, Biochem. Biophys. Res, Commun, 185, N.o 3, 960-966 (1992).2. L. Calza, L. Giardino, and S, CeccateUi, Neuroreport, 4, No-4, 627-630 (1993). 3. R. G. Knowles, M. Palacios, R. M. Palmer, and S.Moncada, Biochem. J., 269, 207-210 (1990 Neuroreport, 4, No-2, 267-270 (1993). Translated from Byulleten'Eksperimental'noi Biologii i Meditsiny, Vol.l17, No 1, pp.41-44, January, 1994 Original article submitted July 5, 1993 In contrast to short-term exposure, prolonged exposure to ethanol in the antenatal period is found to inhibit lipid peroxidation in the brain and liver of rats. Activation of the system of antioxidant defense in the brain and liver is observed after both shortand long-term exposure to ethanol. After short-term ethanol exposure, limontar norrealizes lipid peroxidation. Antenatal Effects of Ethanol and Limontar on LipidKey Words: antenatal effect of ethanol; lipid peroxidation; enzymatic system of antioxidant defense; brain; liver Lipid peroxidation (LPO) activated by ethanol impairs biological membranes and their permeability [10,12]. The enzymatic system of antioxidant (AO) defense also responds to ethanol administration

Currently, in the diagnosis of diseases, a decisive place is given to laboratory methods, which should be informative, relatively simple to perform and rapid. The article describes the approbation of a method for rapid detection of Escherichia coli and bacteria of Escherichia coli group in the oral cavity. Research involved 44 volunteers, who were sampled from the oral cavity, followed by incubation in Koda’s medium. The study used oral (n=11) and gingival fluids (n=11); smears-prints from the oral mucosa (n=11); dental biofilm (n=11). After 24 hours, the change in color and transparency of the medium was assessed. The preservation of the initial green color and transparency by the medium meant the absence of E. coli and bacteria of Escherichia coli group in the sample. A change in the color of the medium to yellow, turbidity and / or the formation of bubbles indicated the presence of E. coli and bacteria of Escherichia coli group. In parallel, the material was inoculated onto Endo agar, followed by identification of strains to species. As a result of the study, a complete coincidence of the results of the classical bacteriological method and the method using Koda medium was shown. In the latter case, a significant advantage is the speed of obtaining the result (18-20 hours), in contrast to the classical method, the interpretation of the results of which is available only after 72 hours or more. All of this is in line with the state of the art in clinical microbiology and rapid diagnosis based on «point-of-care testing / doctor’s office» diagnostic principle. The presented method can be successfully applied in clinical practice for topical diagnosis of microorganisms E. coli and bacteria of Escherichia coli group in the oral cavity.

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