Myeloperoxidase (MPO) is an unequivocal marker of myeloid differentiation which is routinely detected using cytochemistry (CC), flow cytometry (FC) and immunohistochemistry (IH). Several studies have compared the use of two of these methods, but to our knowledge none has compared all three techniques. We compared the performance of these three modalities in the detection of MPO in 158 cases of acute myeloid leukaemia (AML). Discrepancies were noted in a total of 28 cases. Of 110 cases in which all three modalities were performed, 23 cases showed discrepancies. CC was the least sensitive marker, being negative in 11 of 23 cases in the presence of positive IH and/or FC. IH was the most sensitive marker with only one case being negative in the presence of a positive result by FC and/or CC. The results highlight the necessity of employing more than one method in determining the presence of MPO and confirm the important role of IH in the diagnosis of AML particularly in cases where MPO is not detected by CC and FC.

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The investigation was carried out to study the effect of IBA at different concentrations (2000ppm, 4000ppm, 6000ppm) on cuttings of dragon fruit cultivars viz., red pulped variety and white pulped variety to elicit their rooting and shooting parameters. The cuttings of the red pulped variety performed better than the white pulped variety. Indole butyric acid (IBA) had significant effect on rooting and shooting performance of both the varieties over control. IBA @ 6000 ppm recorded minimum number of days for sprouting (43days), maximum rooting and shooting parameters like percentage of rooted cuttings (86.77%), number of roots (9.87), length of longest root (22.93cm), root fresh weight (1.83 g) and dry weight (0.58 g), length of the longest shoot (23.93cm), number of new shoots per cutting (4.00), shoot fresh weight (112.09 g), shoot dry weight (9.47 g) and survival percentage (90.26 %). The least performance was observed in the cuttings of white pulped variety for almost all parameters tested except for length of shoot.

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