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Stock cultures were maintained at 4°C on slopes of nutrient agar. Active cultures for experiments were prepared by transferring a loopful of cells from the stock cultures to test tubes of Mueller-Hinton Broth (MHB) for bacteria and Sabouraud Dextrose Broth (SDB) for fungi that were incubated without agitation for 24 h at 37°C and 25°C respectively. The cultures were diluted with fresh Mueller-Hinton and Sabouraud dextrose broth to achieve optical ABSTRACT Antimicrobial activity of an ethanolic polyherbal extract was examined against the bacterial strains, Escherichia coli, Proteus mirabilis, Gardnerella vaginalis and Pseudomonas aeruginosa and the fungal strains, Candida albicans and Aspergillus niger using disc diffusion method. The polyherbal extract was prepared from the combined ethanolic extracts of Saraca indica, Symplocos racemosa, Hemidesmus indicus, Aloe vera, Asteracantha longifolia, Erythrina indica and Tribulus terrestris. Antibacterial activity was determined using Mueller Hinton Agar (MHA) medium and antifungal activity was studied using Potato dextrose agar (PDA) medium. The polyherbal extract showed significant antimicrobial activity against the tested microorganisms. The minimum inhibitory concentration (MIC) value was also determined which was found to be 1 mg/ml against the selected microorganisms.

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