Host trees are immediate environments for multi‐host mistletoes, so parasitizing different hosts implies exposure to varying environmental conditions between mistletoe generations. Therefore, to maximize reproductive fitness in heterogeneous environments (host species) mistletoes should adjust its reproductive responses in relation to circumstances. Here, we ask how reproductive traits of Psittacanthus calyculatus mistletoes vary on two host tree species (Crataegus mexicana, Prunus serotina) at three different localities. We followed individual mistletoes on each host species and at three localities through the flowering season to quantify traits related to reproductive effort and success, e.g. total number of buds, flowers and fruits produced. In buds about to open, we measured two anthers and quantified the number of pollen grains and their viability. Individual flowers were marked to obtain flower longevity data and were followed until fruit formation. At which time we measured and weighed the fruits and the seeds. Mistletoes from one locality produced more buds, flowers and fruits, and the flowers lasted longer, had larger anthers and produced more pollen as compared to the other two localities. However, mistletoes on Prunus serotina produced fewer floral buds, but their fruits were heaviest, longest and widest and ripened fastest across localities. The probability of fruit formation, percentage of fruits formed, and pollen viability were similar among the mistletoes, regardless of host species or locality. We propose that the observed differences in reproductive effort and success associated with host species or locality are plastic or adaptive in this mistletoe in response to varying conditions.