Environmental DNA (eDNA) metabarcoding is a promising approach to identify species within communities and can be used to evaluate biodiversity through a variety of estimators (Boulanger et al., 2021;Deiner et al., 2020;Pawlowski et al., 2018). The approach is based on the collection of environmental samples (e.g., soil, air or water) that contain the target organisms' DNA. After DNA extraction, DNA amplification with primers designed for a specific taxonomic group is performed and submitted to high-throughput sequencing (Deiner