Background: Human papillomavirus (HPV) is a group of common viruses that can cause a sexually transmitted infection (STI). People with HPV infection can be susceptible to cervical cancer, anogenital warts, and another HPV-related disease. Although the HPV infection can be prevented by HPV vaccination, the vaccination rate in Hong Kong is relatively low particular in males comparing to other countries. Study design: An exploratory qualitative approach was adopted. Aim: This study attempted to explore the perceptions of male college students refusing HPV vaccination in Hong Kong. Methods: A total of 20 male college students participated in the unstructured interview with audiotape recording and field-notes done to explore their perceptions refusing HPV vaccination. Content analysis was utilized to extract significant themes and sub-themes. Results: Six major themes were identified including a lack of perceived risk, knowledge deficit, inadequate promotion, a high price of the HPV vaccine, inconvenience to receive the HPV vaccine and peer influence. Conclusion: Exploring the perceptions for Hong Kong male college students refusing HPV vaccination could provide essential information to the local government to establish corresponding health campaign for males to increase receiving HPV vaccination for curbing the HPV-related diseases.