This study aims to develop and validate a biological food preference task that simultaneously evaluates biological responses to visual stimuli of various food states and subjective evaluations of foods and to examine how these biological responses are related to food preference behavior. We recruited seventeen healthy male and female subjects to observe changes in cerebral blood flow related to salivation and the prefrontal cortex region while performing a food preference task related to visual stimuli of various food states. We also examined the relationship between these changes and the subjects' subjective evaluations. The results showed that subjective evaluations of the various states of visual stimuli differed from subjective evaluations of the different food states. Furthermore, comparing the hemodynamic response function of cerebral blood flow to each visual stimulus, we observed a trend of activation of brain activity in the prefrontal and parotid regions during task execution. In addition, correlations were calculated between the subject's subjective evaluation and cerebral blood flow in the prefrontal and parotid regions and between cerebral blood flow in the prefrontal and parotid regions, and significant differences were observed. Our findings demonstrate the potential of combining the evaluation of food in different states with cerebral blood flow indices in biological responses to visual cues of food.