To test the adjunctive effect of 0.1% sodium hypochlorite (NaOCl) mouthwash combined to full‐mouth ultrasonic debridement (FMUD) on reducing supragingival plaque, gingival inflammation, and microbial pathogens. In this 6‐month double‐blinded randomized clinical trial, individuals with gingivitis were assigned to test (n = 16) or placebo group (n = 16) and received FMUD followed by rinsing with 0.1% NaOCl (test) or distilled water (placebo), respectively, twice a day for 1 month. Full‐mouth periodontal examination was performed at baseline, 1, 3, and 6 months posttherapy, and subgingival plaque samples were obtained at the same time points and analysed for their composition by checkerboard. Differences between groups over time were examined by Student t test, Mann–Whitney, generalized linear model, and Friedman and chi‐square tests. Both therapeutic protocols resulted in significant clinical improvement in periodontal parameters over time, except for probing depth and attachment level, which had a slight mean increase of 0.2 mm (p < .01). No significant differences between groups were observed for any clinical parameter (p > .05). Most species (>65%) decreased similarly in levels in both groups over time. Significant reductions in the microbial complexes were seen mainly at 1 and 3 months, but they returned to baseline levels in both groups, except for the red and yellow complexes, and other oral species, which were kept in low levels at 6 months (p < .05). A 0.1% NaOCl mouthwash did not provide additional benefits to FMUD in reducing supragingival plaque, gingivitis, and/or microbial pathogens.

Background: This cross-sectional study aimed to determine the prevalence and antimicrobial susceptibility of Gram-negative bacilli (GNB) isolated from subgingival biofilm of individuals with different periodontal conditions. Methods: Subgingival biofilm was obtained from 362 individuals with periodontal health (PH) (n = 83), gingivitis (n = 74), and periodontitis (n = 205), cultivated in broth and selective media. Isolated strains were identified by mass spectrometry. Antimicrobial susceptibility was determined by the Clinical and Laboratory Standards Institute disk diffusion guidelines. Production of extendedspectrum beta-lactamase (ESBL) and carbapenemases were evaluated by double disk synergy test and spectrophotometric detection of imipenem hydrolysis, respectively. ESBL and carbapenemase encoding genes were surveyed by Polymerase chain reaction (PCR). Differences among groups were examined by Chisquare, Kruskal-Wallis or Mann-Whitney tests. Results: GNB were isolated from 36.2% of all subgingival biofilm samples, with a significantly greater prevalence and species diversity (P < 0.001) in patients with periodontitis (45.9%) compared with individuals with PH (24.1%) and gingivitis (22.9%). Pseudomonas aeruginosa (27.5%), Enterobacter cloacae (16.8%), and Enterobacter asburiae (10.7%) were the most predominant species. Resistance/reduced sensitivity to at least 1 antimicrobial was detected in 60% of the strains, but only 4.6% were multidrug resistant. Serratia marcescens, E. cloacae, and Enterobacter kobei presented high rates of intrinsic resistance (>40%) to amoxicillin-clavulanate and first/second-generations of cephalosporins. One strain of Klebsiella pneumoniae isolated from periodontitis was resistant to imipenem, but no ESBL encoding genes or ESBL phenotype was detected. Conclusion: High prevalence and diversity of GNB, with low susceptibility to βlactams are observed in the subgingival microbiota associated with periodontitis.

Background and objectives: Periodontitis can corroborate with development and progression of atherosclerosis and a possible bidirectional interaction between both pathologies has been hypothesized. The aim of this work was to study the interactions between diet-induced hypercholesterolemia and ligature-induced periodontitis in Wistar rats submitted to both conditions. Material and methods: Animals were divided into four experimental groups: C (control: standard diet without periodontitis), Perio (periodontitis plus standard diet), HC (high cholesterol diet without periodontitis), and HC + Perio (high cholesterol diet plus periodontitis). The diets were offered for 45 days and a silk ligature was applied in the lower first molars of Perio and HC-Perio animals on day 34 and maintained for 11 days until euthanasia. The mandibles were excised, and alveolar bone loss was determined by macroscopic and micro-tomographic (µ-CT) imaging. Blood samples were obtained, and platelet aggregation was induced in plasma rich in platelets by adenosine diphosphate (ADP) and collagen. Endothelium-dependent vascular reactivity and protein expression of endothelial (eNOS), phosphorylated endothelial (peNOS), and inducible (iNOS) nitric oxide synthases were evaluated in aorta samples. Results:The HC diet combined with periodontitis (HC + Perio group) was associated with an increased alveolar bone loss, when compared to the other groups. Both in Perio and HC groups, platelet aggregation induced by ADP or collagen was increased, while maximum aortic relaxation induced by acetylcholine was decreased.Periodontitis or HC diet alone decreased the expression of peNOS and HC diet increased the expression of iNOS. In contrast, no additive or synergistic effects were found in vascular reactivity or in platelet aggregation when the two conditions were associated (HC + Perio group). Conclusion:Hypercholesterolemia accelerated the process of bone loss induced by periodontitis while a high cholesterol diet or periodontitis individually increased

Background: This study evaluated the prevalence, virulence and antimicrobial susceptibility of enterococci isolated from the subgingival microbiota of patients with different periodontal status. Methods: Subgingival biofilm was obtained from individuals with periodontal health (PH) (n = 139), gingivitis (n = 103), and periodontitis (n = 305) and cultivated on selective media. Isolated strains were identified by mass spectrometry.Antimicrobial sensitivity was determined by disk diffusion, virulence genes by polymerase chain reaction, and the subgingival microbiota by checkerboard. Differences among groups were assessed by Kruskal-Wallis, Mann-Whitney, and Chi-square tests. Results: Enterococcus spp. were isolated from 7.4% of all samples; 53.7% were Enterococcus faecalis. They were more prevalent in periodontitis (9.8%) and gingivitis (7.8%) than PH (2.2%; P <0.05), but no differences among stages of disease severity were observed. High rates of low susceptibility/resistance (>64%) to at least one antimicrobial were observed. Predominant virulence factors included ace (64.3%), asa (39.3%), and esp (35.7%). Fusobacterium nucleatum was prevalent in the subgingival microbiota of enterococci+ individuals, whereas Dialister pneumosintes was found in low frequency in patients with bopD+ enterococci.Oral streptococci were prevalent (>70%) in patients carrying enterococci susceptible to doxycycline (P <0.05), usually bopDand esp-(P <0.01).Conclusions: E. faecalis is increased in periodontitis-associated biofilm. Oral enterococci carry virulence genes and express resistance to some antibiotics commonly used in dentistry, such as ciprofloxacin and erythromycin. Specific subgingival taxa are associated with oral enterococci, suggesting they may interact with species of the dysbiotic periodontitis biofilm, constituting a potential source of factors to tissue destruction, antibiotic resistance dissemination, and poor response to periodontal therapy.

O tratamento de dentes com rizogênese incompleta e necrose pulpar representa um grande desafio para a terapia endodôntica, principalmente pela fragilidade das paredes radiculares desses dentes. Uma terapêutica que pode ser realizada é a revascularização pulpar com o objetivo de substituir estruturas danificadas, incluindo estruturas dentinárias e radiculares, bem como células do complexo dentino-pulpar com um tecido que permita a continuação da formação da raiz em espessura e tamanho. Esses procedimentos demonstraram ser capazes de promover o desenvolvimento continuado da raiz, aumentando assim a espessura e a resistência das raízes anteriormente finas e propensas à fratura. Este trabalho tem como objetivo realizar, através de uma revisão da literatura, um estudo sobre a revascularização pulpar e sua importância clínica no tratamento de dentes acometidos por necrose pulpar e cuja formação apical ainda não se completou. Para este estudo, foi realizado um levantamento na plataforma PubMed, em que foram incluídos artigos publicados entre 2004 e 2021.  A revascularização pulpar pode ser considerada como uma alternativa promissora como tratamento para dentes com rizogênese incompleta e necrose pulpar, tendo vantagens potenciais em relação ao tradicional processo de apicificação.