Action of disinfectant solutions on adaptive capacity and virulence factors of the Candida spp. biofilms formed on acrylic resin Understanding the behavior of Candida spp. when exposed to denture disinfectants is essential to optimize their effectiveness. Changes in the virulence factors may cause increased resistance of Candida spp. to disinfectant agents. Objective: To evaluate the microbial load, cellular metabolism, hydrolytic enzyme production, hyphae formation, live cell and biofilm quantification of Candida albicans, Candida tropicalis and Candida glabrata after exposure to disinfectant solutions. Methodology: Simple biofilms were grown on heat-polymerized acrylic resin specimens, and divided into groups according to solutions/strains: distilled water (control); 0.25% sodium hypochlorite (NaOCl 0.25% ); 10% Ricinus communis (RC 10%); and 0.5% Chloramine T (CT 0.5%). The virulence factors were evaluated using the CFU count (microbial load), XTT method (cell metabolism), epifluorescence microscopy (biofilm removal and live or dead cells adhered), protease and phospholipase production and hyphae formation. Data were analyzed (α=0.05) by one-way ANOVA/ Tukey post hoc test, Kruskal-Wallis test and Wilcoxon test. Results: NaOCl 0.25% was the most effective solution. CT 0.5% reduced the number of CFUs more than RC 10% and the control. RC 10% was effective only against C. glabrata. RC 10% and CT 0.5% decreased the cellular metabolism of C. albicans and C. glabrata. Enzyme productionwas not affected. Hyphal growth in the RC 10% and CT 0.5% groups was similar to that of the control. CT 0.5% was better than RC 10% against C. albicans and C. tropicalis when measuring the total amount of biofilm and number of living cells. For C. glabrata, CT 0.5% was equal to RC 10% in the maintenance of living cells; RC 10% was superior for biofilm removal.Conclusions: The CT 0.5% achieved better results than those of Ricinus communis at 10%, favoring the creation of specific products for dentures.Adjustments in the formulations of RC 10% are necessary due to efficacy against C. glabrata. The NaOCl 0.25% is the most effective and could be suitable for use as a positive control.

Purpose This prospective study evaluated and correlated the impact of ocular prostheses on quality of life and stress with socioeconomic level and clinical characteristics. The clinical difficulties and perceptions of patients after ocular rehabilitation were evaluated. Materials and Methods Anophthalmic patients (at least 18 years of age) who were not users of ocular prostheses were recruited. The Medical Outcomes Short‐Form Health Survey (SF‐36) and Perceived Stress Scale (PSS‐10) questionnaires were administered before and after 3 and 6 months of prosthesis installation. Clinical characteristics, difficulties, and perceptions were evaluated by quantitative and descriptive analysis (7 days, 3 months, 6 months). Data were analyzed by the Friedman test and Pearson Correlation test (α = 0.05). Results The final sample consisted of 26 patients. Quality of life showed improvement in the “Bodily Pain” and “General Health” domains. Wearing the prosthesis did not influence perceived stress. The clinical evaluation showed clinical discharge over 6 months and presence of pain only at 7 days. A weak correlation occurred between sociodemographic characteristics and the categories “Role‐Physical” (r = 0.423) and “General Health” (r = 0.494); cause of anophthalmia and “Role‐Physical” (r = –0.471); and type of surgery and “General Health” (r = –0.432). Conclusions According to the results of this study, the provision of ocular prostheses showed positive influence in 2 domains of quality of life and weak correlations with socioeconomic level, type of surgery, and cause of loss. Ocular rehabilitation did not influence stress. The use and care of the prostheses did not affect tissue inflammation, but the discharge was continuous.

Dedico este trabalho À Deus, por ter me proporcionado grandes oportunidades, por ter protegido o meu caminho e ter me guiado para escolher com sabedoria e decidir o com o coração o que quer que fosse necessário, e principalmente, por ter incluído pessoas tão maravilhosas e amáveis na minha vida. À minha amada mãe Márcia, minha heroína, que com todo seu carinho e proteção de mãe coruja me acolheu e socorreu nos momentos mais difíceis. Sua força e sabedoria me encorajaram a desbravar o mundo e a encarar e arriscar nas oportunidades sem medo.Ao meu querido pai, que com toda sua criatividade e sabedoria me proporcionou uma vida cheia de conhecimento e de curiosidade. Seu carinho e suas peculiaridades foram de grande influência para eu ser quem sou hoje.À minha avó Maria de Lourdes, a quem tenho muito respeito, admiração e gratidão pelo grande apoio proporcionado em toda a minha vida acadêmica. Persona única, forte e destemida, e com um coração misericordioso.Às minhas irmãs Narayna, Eveline e Isadora, por terem cuidado de mim quando pequena e terem me proporcionado uma infância feliz e risonha. Vocês foram, são e sempre serão minhas melhores amigas, minhas conselheiras, minhas protetoras.Aos meus melhores e mais incríveis amigos, os quais tenho muito carinho, apreço e gratidão: Amanda, Flávia, Gabriela, Lucas e Thiago. Vocês foram meus companheiros, meus heróis nos momentos mais difíceis da vida e da graduação. Vocês são a joias mais raras que a vida pode nos oferecer!! Obrigada!! Ao meu amado e carinhoso namorado Luís, que apareceu de forma tão breve e inesperada em minha vida, no momento em que eu mais estava desacreditada. Seu companheirismo, essência e bondade me fazem querer ser alguém melhor e mais em paz comigo mesma. Obrigada por me amar do jeito que sou. Obrigada por ter iluminado minha vida com todo seu amor.

Surgical rehabilitation with grafts and plates of an oncological patient submitted to segmental mandibulectomy may be limited due to the majority receiving late diagnosis, requiring rapid intervention with association of drug therapies and thus, the risk of osteonecrosis. We detail a dental prosthetic rehabilitation in a patient who underwent a segmental mandibulectomy without plates or grafts. Man, 47-years old, affected by a squamous cell carcinoma on the floor of the mouth, treated by segmental mandibulectomy, radiotherapy and chemotherapy, was attended to a dental prosthetic rehabilitation over the mandible segments with a removable partial denture. Initially, a temporary prosthesis was made, in order to adapt the patient with the restoration of the occlusion and recovering the muscle tone. The patient used the temporary removable partial denture for 6 months, accompanied by physical therapy to maintain occlusal stability and regain muscle tone. After this period, it was possible to make a removable partial denture for definitive rehabilitation. The patient proved to be adapted to the prosthetic device and satisfied with the treatment. Prosthetic rehabilitation is a viable option when surgical reconstruction is contraindicated or can be used until the surgical procedure can be realized.