Bioremediation is an important technology to remediate the chromium (Cr) contaminated soil and water. In this study, Shewanella putrefaciens (MTTC8410) was used to investigate the influence of carbon concentration, pH, and temperature on reduction of hexavalent chromium [Cr(VI)] into trivalent chromium [Cr(III)]. The increased bacterial growth rate was significantly reduced the Cr(VI) concentration. In batch mode experiments, 1% starch recorded the highest reduction of Cr(VI) (90%) followed by 1% glucose (88% reduction) and a reduction of 77% was by 1% cellulose. By using various pH conditions the maximum Cr(VI) reduction was achieved at pH 7.0. In this experiment the maximum Cr(VI) reduction (75%) was observed at 35°C, followed by 30°C with 62% of Cr(VI) reduction. Bioreactor analysis revealed the highest reduction of Cr(VI) (88%) in unsterile tannery effluent. The significant levels of physico- chemical parameters were reduced in unsterile tannery effluent, as compared to the sterile tannery effluent. The experimental results revealed that the S. putrefaciens (MTTC8410) could be used as a potential bacterial strain for reduction of Cr(VI) from contaminated groundwater.