Li2TiO3 nanopowders were synthesized by hydrothermal process using anatase TiO2 and LiOHH2O as raw materials. Li2TiO3 crystallizes in the layered monoclinic structure (space group C2/c) with average crystallite size of 34 nm. Morphology, elemental composition and local structure of products were carried out using high-resolution transmission electron microscopy, field-emission scanning electron microscopy, Raman and Fourier transform infrared spectroscopy. Transport properties investigated by d.c. (4-probe measurements) and a.c. (complex impedance spectroscopy) show the activation energy of 0.71 and 0.65 eV, respectively. The ionic transport properties of Li+ ions in nanocrystalline Li2TiO3 characterized by cyclic voltammetry and impedance spectroscopy validate the good electrochemical properties of this anode material for lithium-ion batteries.