High blood pressure is the largest single risk factor for premature death. As it is known, some patients with hypertension require two or more antihypertensive drugs with complementary mechanisms of action to lower their blood pressure. The angiotensin II type 1-receptor antagonist valsartan and the diuretic hydrochlorothiazide ( Fig. 1) are two antihypertensive agents with well recognised clinical efficacy. Oral administration of valsartan with hydrochlorothiazide has been found to be more effective than either drug alone in the treatment of hypertension in patients whose blood pressure is not Calibration matrices contained 0.5-3 µg mL -1 of both valsartan and hydrochlorothiazide. The standard error of prediction (SEP) for valsartan and hydrochlorothiazide was 0.020 and 0.038 mg mL -1 , respectively. Both proposed methods were successfully applied to the determination of valsartan and hydrochlorothiazide in several synthetic and real matrix samples.

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A simple, sensitive and validated HPLC method has been developed to determine valsartan and ramipril simultaneously in synthetic mixture. Chromatographic separation was achieved on a C-18 column using a mixture of acetonitrile and water in the ratio 55:45 (v/v), pH adjusted to 3.6 with 88% orthophosphoric acid at a wavelength of 215 nm. Linearity of the method was found to be in the concentration range of 50-250 µg/ml for valsartan and100-500 µg/ml for ramipril with correlation coefficient greater than 0.999. The total eluting time for the two components is less than five minutes. The method can be used for simultaneous determination of valsartan and ramipril.

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