Drug resistance is a serious medical problem. Indiscriminate use of antibiotics has led to a state where multi-drug resistant bacteria have become increasingly prevalent. Therefore regular surveillance of important pathogens and their resistant pattern is mandatory. AIMTo find out prevalence of organisms causing infection and their sensitivity pattern. MATERIAL AND METHODS676 clinical samples were screened, among which 156 Gram Negative (GN) Isolates were processed for their antibiotic sensitivity profile against 12 different antibiotics. RESULTSEscherichia coli is the most common isolate of 156 gram negative isolates. Among all antibiotics, Ampicillin is least sensitive (22%). Antibiotics with good sensitivity are Imipenem, Meropenem (100%), Levofloxacin 94%, Amikacin 89%, Ciprofloxacin 79%, Gentamicin 77%. Pseudomonas is 100% sensitive to Amikacin. CONCLUSIONAntibiotic resistance in our area is still moderate. It is essential to test for older generation antibiotics before deciding on higher antibiotics for treatment, which will have a tremendous impact on the treatment as well as cost effectiveness. Regular surveillance helps in implementing better therapeutic strategies.