The objective of this study was to characterize biogas production performance from the co-digestion of food waste and domestic wastewater under mesophilic (35 ± 1 °C) and thermophilic (55 ± 1 °C) conditions. The food waste used as a co-substrate in this study was collected from a main canteen at the Hatyai campus of Prince of Songkla University, Songkhla Province, Thailand. The optimum co-digestion ratio and temperature conditions in a batch experiment were selected for a semi-continuous experiment. Organic loading rates (OLRs) of 0.66, 0.33, and 0.22 g volatile solid (VS) L−1 d−1 were investigated in a semi-continuous experiment by continuously stirring a tank reactor (CSTR) for biogas production. The highest biomethane potential (BMP, 0.78 ml CH4 mg−1 VS removal) was achieved with a ratio of food waste to domestic wastewater of 10:90 w/v at a mesophilic temperature. An OLR of 0.22 g VS L−1 d−1 of co-digestion yielded positive biogas production and organic removal. The findings of this study illustrate how biogas production can be used for operating feed conditions and control for anaerobic co-digestion of domestic wastewater and food waste from a university canteen.