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BackgroundSoybean grain is an important oil crop with high-quality vegetable protein and vegetable oil. Extreme weather can cause crop yield reduction, among which drought is most likely to cause a decline in annual soybean yield. How to overcome the impact of drought on soybean yield has become a major work in current breeding research.ResultsIn our study, the gene GmELF4-LIKE4 was obtained through RNA-Seq and differential gene screening technology, and the plant over expression vector and RNAi vector were constructed. Then, the Colombian Arabidopsis thaliana and soybean JN18 were genetically transformed and added to the Agrobacterium-mediated method in to T2 generation. We have verified in Arabidopsis thaliana that over expression of ELF4-LIKE4 will delay the flowering of Arabidopsis thaliana, while inhibition of ELF4-LIKE4 expression will advance the flowering period. At the same time, we verified the regulation analysis of GmELF4-LIKE4 on EARLY FLOWERING 3 and CONSTANTS-like in soybeans, and found that GmELF4-LIKE4 positively regulates ELF3 and COL; meanwhile, over expression soybeans and RNAi soybeans were tested in drought. Stressing the relative conductivity, malondialdehyde content and peroxidase activity under 0 days and 5 days, the result indicate that the over expression of GmELF4-LIKE4 gene can reduce the drought resistance of soybean. This study laid a theoretical foundation for the identification of GmELF4-LIKE4 gene function and the breeding of drought-resistant varieties.ConclusionWe found that overexpression of GmELF4-LIKE4 will delay the flowering period of Arabidopsis thaliana and reduce the drought resistance of soybeans. Conversely, inhibiting GmELF4-LIKE4 will advance the flowering of Arabidopsis thaliana and increase the drought resistance of soybeans.

High yield, high quality, stable yield, adaptability to growth period, and modern mechanization are the basic requirements for crops in the 21st century. Soybean oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and stability. Known as a safe fatty acid, it has the ability to successfully prevent cardiovascular and cerebrovascular disorders. Improving the fatty acid composition of soybean seeds, can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans, but also have important significance in human health, and provide the possibility for the development of soybean oil as a new energy source. Hence, the aim of this study was to analyze the high oleic acid elated gene GmSAM22 in soybean. In this research the soybean oleic acid-related gene GmSAM22 was screened out by Genome-wide association analysis, a 662 bp fragment was acquired by specific PCR amplification, and the pMD18T cloning vector was linked by the use of a seamless cloning technique. Bioinformatics analysis of the signal peptide prediction, subcellular localization, protein hydrophobicity, transmembrane region analysis, a phosphorylation site, protein secondary and tertiary structure and protein interaction analysis of the protein encoded by the SAM22 gene was carried out. The plasmid of the gene editing vector is pBK041. The overexpression vector was transformed from pCAMBIA3301 as the base vector, and overexpression vector were designed. Positive plants were obtained by genetic transformation by the pollen tube channel method. Fluorescence quantitative PCR was performed on the T 2 generation plants to detect the relative expression levels in different tissues. Southern Blot was used to detect the presence of hybridization signal. Screening genes BAR, 35S, and NOS in plants were identified by conventional PCR. 10 seeds with high and low oleic acid content were chosen for quantitative PCR identification, and finally, the concentration and morphology of soybean fatty acids were identified by nearfar infrared spectroscopy. On 10 seeds with an upper and lower oleic acid content, a quantitative fluorescence analysis was done. In Southern blot hybridization, the SAM22 gene was integrated into the recipient soybean plant in hands of a sole copy. Fluorescence quantitative PCR appeared that the average relative expression of the SAM22 gene in roots, stems, leaves, and seeds was 1. 70, 1.67, 3.83, and 4.41, respectively. Positive expression seeds had a 4.77% increase in oleic acid content. The level of oleic acid in the altered seeds was reduced by 4.13% when compared to CK, and it was discovered that the GmSAM22 gene could be a regulatory and secondary gene that promotes the conversion of stearic acid to oleic acid in soybean. There has not been a discussion of gene cloning or functional verification. The cloning and genetic transformation of the soybean SAM22 gene can effectively increase the content of oleic acid, which lays a foundation for the study of soybean with high oleic acid.