Rheumatoid arthritis (RA) is characterized by inflammation of one or more joints, and affects ~1% of the adult population worldwide. Sulforaphane (SFN) is a natural compound that has been suggested as an antioxidant. Here, SFN’s effects were evaluated in a murine mono-arthritis model. Mono-arthritis was induced in mice by a single intra-articular injection of Complete Freund’s Adjuvant (CFA-10 µg/joint, in 10 µL) into the ipsilateral joint. The contralateral joint received an equal volume of PBS. On the 4th day post-joint inflammation induction, animals received either SFN (10 mg/kg) or vehicle (3% DMSO in saline), intraperitoneally (i.p.), twice a day for 3 days. Joint swelling and secondary mechanical allodynia and hyperalgesia were evaluated over 7 days post-CFA. After this period, animals were culled and their blood and synovial fluid samples were collected for analysis of cell populations, cytokine release and thioredoxin reductase (TrxR) activity. Knee joint samples were also collected for histology. SFN reduced joint swelling and damage whilst increasing the recruitment of Ly6C+ and Ly6G+ cells to CFA-injected joints. SFN-treated animals presented down-regulation of CD11b and CD62L on synovial fluid Ly6G+ cells. Synovial fluid samples obtained from CFA-injected joints and plasma samples of SFN-treated mice presented higher levels of IL-6 and increased activity of TrxR, in comparison with controls. These results indicate that SFN reduces knee joint damage by modulating cell activation/migration to the joints, cytokine production and increasing the activity of TrxR, and therefore, may represent an alternative treatment to joint inflammation.

OBJECTIVE To evaluate the knowledge and biosafety practices adopted by professionals of the beauty segment. METHODS Descriptive study, of survey type. 238 professionals of the beauty segment were interviewed between August 2014 and 2015. The variables were expressed by means of absolute and relative frequencies, as well as average and standard deviation. RESULTS 62.6% of the interviwed professionals reported having had contact with blood from customers when they were not wearing gloves; 74.4% said they washed their hands before and after each service, and only 16.8% of the respondents reported reusing nonsterilizable materials. None of them was able to inform the correct number of sets of tools needed , and 32.8% of the respondents did not use Personal Protective Equipment during their work activities. CONCLUSIONS The most frequently reported diseases associated with the risk of infection and transmission in the work activities were viral hepatitis, HIV and fungi. Regarding the biosafety procedures adopted, autoclave is the least used method for sterilizing devices.

Objetivo: Refletir teoricamente acerca da Atenção Primária em Saúde e as Redes de Atenção à Saúde quanto à articulação entre ambas para o enfrentamento da Covid-19. Método: Estudo reflexivo, embasado na formulação discursiva sobre a APS e as RAS no contexto pandêmico. Resultados: Telemedicina, acompanhamento de casos suspeitos e confirmados, criação de novos fluxos assistenciais e medidas de articulação entre as redes foram necessárias a APS, no contexto das RAS, para enfrentamento à pandemia. Este cenário reforçou a necessidade da articulação dos serviços de saúde em redes de atenção, com vistas a oferta de cuidado integral à população. Conclusão: Torna-se clara a importância da APS para o cuidado integral dentro de um sistema de saúde. Em um cenário caótico, incerto, com rápido crescimento de indicadores de incidência e mortalidade, a APS teve que se reorganizar e a partir disso desempenhar seu papel de ordenadora e coordenadora das RAS.

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Objetivo: conhecer a percepção de enfermeiros da Atenção Primária sobre plantas medicinais e fitoterápicos. Métodos: estudo qualitativo realizado em Unidades de Saúde da Família no município de Pinheiro-MA, Brasil, no período de fevereiro a maio de 2022. Resultados: Observou-se baixo nível de conhecimento científico sobre a temática, relacionado à ausência de aprendizado significativo sobre o assunto durante a graduação e no período de atuação profissional, estando o pouco conhecimento que possuem condicionado a saberes familiares. A falta de qualificação adequada é a principal dificuldade para a aplicação da fitoterapia na assistência à saúde, apesar de reconhecerem a relevância dessa prática por seu baixo custo, eficácia e fácil acesso da população. Conclusão: Evidenciou-se pouco embasamento quanto à fitoterapia e plantas medicinais pelos profissionais, revelando a necessidade de criação de estratégias de ação voltadas à inserção da fitoterapia na Atenção Primária do município.