: Several strategies have been tried out for the control and antimicrobial treatment of carious lesions such as the direct application of ozone. However, the the oxidation process after the use of ozone facilitates the permanence of residual oxygen, which can negatively influence the use of adhesive systems. The application of 10% sodium ascorbate on the dentin surface can neutralize the effect of oxygen. However, the influence of these substances on the coronary dentin microhardness must be studied. Objective: This study aimed to evaluate the microhardness of human dentin after the application of gaseous ozone and sodium ascorbate. Material and methods: Nineteen third molars were sectioned in order to separate the occlusal surface from the roots leaving a 4 mm thick specimen which was further divided into its mesial and distal parts. The mesial parts were allocated to Group A which was subdivided into two groups: control group, in which the initial microhardness was measured, and an ozone group, in which the application of gaseous ozone was performed for 40 seconds followed by the measurement of the final microhardness. The distal parts were allocated to Group B, subdivided into two groups, a control group in which the initialmicrohardness was measured and an ozone + sodium ascorbate group in which the application of gaseous ozone was performed for 40 seconds and a solution of 10% sodium ascorbate for 10 minutes followed by measurement of the final microhardness. Results: The results showed that there was a statistically significant increase in dentin microhardness (p>0.05) in Group A (control and ozone) and in Group B (control and ozone + sodium ascorbate). However,there was no statistically significant increase in microhardness (p>0.05) between ozone in group A and ozone + sodium ascorbate in group B. Conclusion: The application of gaseous ozone increased the microhardness of human dentin and the application of sodium ascobate had no influence on the microhardness that has already been modified by gaseous ozone.

Resumo: Considerando a estética anterior do sorriso gengival, o uso de próteses implanto-suportadas tem sido bastante eficaz para reabilitação de espaços protéticos. Entretanto, para um resultado de excelência em estética, a morfologia gengival deve estar harmonizada com o sorriso e a prótese. Este estudo teve como objetivo relatar o caso clínico de uma paciente que foi submetida a exodontia seguida de provisório imediato dos elementos 12 e 22, e posteriormente a exodontia dos elementos 11 e 21, seguido da instalação de prótese provisória múltipla do incisivos superiores. A fim de harmonizar o sorriso, foram trabalhadas algumas sessões de condicionamento gengival, utilizando a técnica de pressão sob a margem gengival através do término da prótese provisória. Para isto, foram coletados imagens e dados em prontuários. Ao final do tratamento notouse uma melhora na arquitetura gengival e perfil de emergência. Logo, conclui-se que o tratamento para condicionamento gengival previamente a prótese definitiva é um passo importante na reabilitação de áreas protéticas, principalmente quando em regiões estéticas.Abstract: Considering the anterior aesthetics of gingival smile, the use of implant-supported prostheses has been very effective for rehabilitation of prosthetic spaces. However, for a result of aesthetic excellence, gingival morphology must be harmonized with smile and prosthesis. This study aimed to report the clinical case of a patient who underwent extraction followed by immediate provisional extraction of elements 12 and 22, and subsequently the extraction of elements 11 and 21, followed by the installation of a temporary multiple incisor prosthesis. In order to harmonize the smile, some gingival conditioning sessions were worked, using the pressure technique under the gingival margin through the completion of the provisional prosthesis. For this, images and data were collected from medical records. At the end of treatment, an improvement in gingival architecture and emergence profile was noted. Therefore, it is concluded that the treatment for gingival conditioning prior to the definitive prosthesis is an important step in the rehabilitation of prosthetic areas, especially when in aesthetic regions.