AbstrakKerapatan kolagen merupakan satu faktor penting dalam kejadian prolaps uteri. Beberapa penelitian menunjukkan pasien prolaps uteri mempunyai kerapatan kolagen ligamentum sakrouterina yang rendah. Penelitian ini bertujuan mengukur perbedaan kerapatan kolagen ligamentum sakrouterina pada pasien dengan dan tanpa prolaps uteri. Penelitian ini adalah penelitian analitik komparatif dengan case control study terhadap 16 pasien prolaps uteri dan 16 pasien tanpa prolaps uteri di Rumah Sakit Dr. Hasan Sadikin Bandung dan RS jejaring periode November-Desember 2008. Bahan penelitian diambil dari ligamentum sakrouterina saat operasi histerektomi lalu dibuat sediaan dengan pewarnaan hematoksilin-eosin dan Masson's trichrome. Uji kemaknaan menggunakan uji Mann-Whitney. Hasil penelitian didapatkan hubungan bermakna antara kerapatan kolagen dan kejadian prolaps uteri, yaitu kerapatan kolagen pada pasien prolaps uteri lebih rendah (15,3%) dibanding dengan tanpa prolaps uteri (48,75%). Cut-off point kerapatan kolagen ligamentum sakrouterina untuk prediksi prolaps uteri adalah ≤ 30%; sensitivitas 93,8%; spesifisitas 87,5%; dan akurasi 90,6%. Simpulan penelitian ini adalah bahwa kerapatan kolagen ligamentum sakrouterina pada pasien prolaps uteri lebih rendah dibanding dengan pasien tanpa prolaps uteri. Cut-off point kerapatan kolagen ligamentum sakrouterina yang dapat memprediksi prolaps uteri adalah ≤ 30%. [MKB. 2015;47(4):212-7] Kata kunci: Kerapatan kolagen, ligamentum sakrouterina, prolaps uteri The Comparison of Uterosacral Ligament Collagen Density in Patients with and without Uterine Prolapse AbstractCollagen density is one important factor in uterine prolapse. Several studies has shown that uterine prolapse patients have lower uterosacral ligament collagen density. The purpose of this study was to reveal the uterosacral ligament collagen density differences in patients with and without uterine prolapse. This case control study was an analitic comparative research of 16 uterine prolapse patients and 16 patients without uterine prolapse who underwent hysterectomy in Dr. Hasan Sadikin General Hospital Bandung and its networking hospitals during November-December 2008. Uterosacral ligament was sampled and then stained using HE and Masson's trichrome staining. The significance of the result was analyzed using Mann-Whitney. The study found a significant correlation between collagen density and uterine prolapse, with the collagen density of uterine prolapse patients was lower (15.3%) than patients without uterine prolapse (48.75%). The uterosacral ligament collagen density cut-off point in predicting women having uterine prolapse was ≤30%; with 93.8% sensitivity, 87.5% specificity, and 90.6% accuracy. The conclusion of this study is that uterosacral ligament collagen density in patients with uterine prolapse is lower (15.3%) than patients without uterine prolapse (48.75%). The uterosacral ligament collagen density cut-off point in predicting a women having uterine prolapse is ≤30%. [MKB. 2015;47(4):212-7]