The crude protein was extracted from marine edible bivalve Donax cuneatus, precipitated and further dialysed against PBS in dialysis membrane having MW cut off of 3 KDa. The extract was evaluated for its antimicrobial activity against highly pathogenic Gram positive and Gram negative bacteria. It was found to be active against Pseudomonas aeruginosa and Staphylococcus aureus. The purified fractions obtained from anion-exchange column were evaluated for their activity against the aforesaid bacteria. The highly active fraction against Staphylococus aureus with the zone of inhibition of 12 mm was identified and further purified and characterised. The active fraction was enriched with glycine, alanine and asparagine aminoacids.

The marine bacterium was widely recognized as a natural manufacturer of novel bioactive secondary metabolites during the last decades, making the marine ecosystems a huge treasure of novel isolates. Marine coastal areas of the southern part of India have huge varieties of ecosystems that produce many marine microbes and organisms which are considered as a perennial source of many potential drug leads. This study focuses on the isolation of marine soil samples collected from the shores of the Bay of Bengal at Kanyakumari, Tuticorin, and Mandapam and followed by the characterization of the novel marine bacterium from that soil sample for the secondary metabolites production with   potential pharmacological  importance. The isolated  pure  culture  from  colonies  was  cultured  and  taxonomically  characterized  by  gram  staining  and  other  supported biochemical tests. Further, from the genetic analysis results through 16s rDNA sequencing and by comparing the nucleotide homology  and  phylogenetic  analysis  of  the  organisms  it  was  confirmed  as  “Paenibacillus  dendritiformis  and  Paenibacillus thiaminolyticus”.  Moreover, it was found that the bacterial isolates produced the inhibitory activity to other bacterial strains including both gram-positive and gram-negative bacteria at a concentration of 32 µl/ml. Thus, the bacterial isolates were found to produce secondary metabolites that comprise antimicrobial components and enzymes of commercial importance. Henceforth, this preliminary study looks promising and may lead to the discovery of potential antibiotics and other bioactive compounds.