Non-invasive sampling techniques are increasingly being used to monitor glucocorticoids, such as cortisol, as indicators of stressor load and fitness in zoo and wildlife conservation, research and medicine. For cetaceans, exhaled breath condensate (blow) provides a unique sampling matrix for such purposes. The purpose of this work was to develop an appropriate collection methodology and validate the use of a commercially available EIA for measuring cortisol in blow samples collected from belugas (Delphinapterus leucas). Nitex membrane stretched over a petri dish provided the optimal method for collecting blow. A commercially available cortisol EIA for measuring human cortisol (detection limit 35 pg ml−1) was adapted and validated for beluga cortisol using tests of parallelism, accuracy and recovery. Blow samples were collected from aquarium belugas during monthly health checks and during out of water examination, as well as from wild belugas. Two aquarium belugas showed increased blow cortisol between baseline samples and 30 minutes out of water (Baseline, 0.21 and 0.04 µg dl−1; 30 minutes, 0.95 and 0.14 µg dl−1). Six wild belugas also showed increases in blow cortisol between pre and post 1.5 hour examination (Pre 0.03, 0.23, 0.13, 0.19, 0.13, 0.04 µg dl−1, Post 0.60, 0.31, 0.36, 0.24, 0.14, 0.16 µg dl−1). Though this methodology needs further investigation, this study suggests that blow sampling is a good candidate for non-invasive monitoring of cortisol in belugas. It can be collected from both wild and aquarium animals efficiently for the purposes of health monitoring and research, and may ultimately be useful in obtaining data on wild populations, including endangered species, which are difficult to handle directly.
When combined with surgical debridement, relatively short courses of parenteral antimicrobial treatment (<1 week) supplemented with oral therapy for an additional 2-3 weeks is usually sufficient antimicrobial therapy for hand or wrist septic arthritis.
The modified Raymond-Roy scale appears to provide reasonable predictive value for treated aneurysm, especially for the clinically more important aspects of retreatment and rebleed rates.
We investigated the role of training-induced knowledge schemas and encoding time on adult age differences in recall. High-plausible (schema coherent) words were recalled better than lowplausible (schema discrepant) words in both age groups. This difference was larger for old adults than for young adults for presentation times ranging from 3 s to lls per word. After equating participants in overall recall (i.e., at 50% correct) by dynamic adjustment of presentation time, old adults again showed a stronger plausibility effect than young adults when recall was above criterion. In a second experiment with self-paced encoding, old adults used more time than young adults only for low-plausible pairs, yet they still remembered fewer of them. In a third experiment, both age groups preferred to imagine high-rather than low-plausible words, but this effect was more pronounced in old adults. The results indicate that, compared with young adults, old adults find it particularly difficult to form elaborative mental images of schema-discrepant information under a wide variety of time constraints during encoding. Results are discussed in relation to explanations based on age-related mental slowing.
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