Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking particle standards and conditions for long-lived imaging over several weeks. Stable localisation precisions within the 10 nm-range were achieved for single virions and in cellulo 2D imaging of centrosomes, as well as their reliable reconstruction in 3D dSTORM.

In recent years, additive manufacturing (AM) technologies have attracted significant interest in many industrial and research fields, particularly in tissue engineering. Printed structures used as physical and bioactive supports for tissue regeneration are becoming increasingly complex so as to mimic natural tissues in order to answer future medical needs. Reproducing the biological environment of a native tissue from the microscopic to the macroscopic scale appears to be the best strategy for effective regeneration. Recent advances in AM have led to the production of scaffolds designed with a high precision. This Review presents results concerning two AM technologies which enable the highest accuracy of scaffold design to be obtained, with a precision down to the nanoscale. The first technique is based on a two-photon polymerization (TPP) process, while the other is based on a direct-writing electrospinning (DWES) system. Here, we present an overview of the fabrication mechanisms, the final scaffold properties, and their applications in tissue engineering. The production of highly resolved structures offers new possibilities for studying cell behavior in a controlled environment and also for adjusting the desired scaffold properties to address current and future needs in tissue engineering. The current technical limitations and future challenges are thus also discussed in this Review.

Integrating peer support workers (PSWs) into mental health community programs for young people has the potential to increase their access to mental health services. However, very little research has focused on the development of training that is relevant for and readily accessible to youth and employees seeking to acquire a general overview of peer support fundamentals as applicable to youth mental health in community settings. This pilot study aimed to document the implementation fidelity of a newly developed youth mental health peer support training and its impacts on participants’ understanding and perceived ability to provide peer support in community-based youth mental health settings. A multiple-informant approach was used to develop the training which is mapped onto the existing theoretical framework for peer support (Mead, Hilton, & Curtis, 2001). A descriptive quantitative and qualitative research design was used and data were collected using self-reported measures. Five participants completed the 16-hour training, which was offered online through videoconferencing. Results showed that, overall, components of the training were implemented with a high level of fidelity to the theoretical framework. Furthermore, participants reported that the training has had a positive impact on their level of preparedness and ability to provide peer support. Despite the limited sample size, this study suggests that the training developed has practical value as an introductory training tool for youth mental health PSWs. Moreover, this study is the first to provide evidence that the theoretical framework selected is relevant to inform such training. L’intégration de pairs aidants dans les programmes communautaires de santé mentale jeunesse a le potentiel d’accroître l’accès des jeunes aux services de santé mentale. Toutefois, très peu d’études se sont penchées sur le développement de formations adaptées et facilement accessibles aux jeunes et employé·e·s souhaitant acquérir une vue d’ensemble des principes fondamentaux du soutien par les pairs applicables en santé mentale jeunesse en milieu communautaire. Cette étude pilote visait à documenter la fidélité d’implantation d’une formation pour pairs aidants travaillant en santé mentale communautaire jeunesse et ses impacts sur la compréhension et la capacité perçue des participant·e·s à fournir un soutien par les pairs. Cette formation a été développée selon une approche multi-informateurs en s’appuyant sur le cadre théorique de la pair-aidance de Mead, Hilton et Curtis (2001). Une méthodologie descriptive quantitative et qualitative a été utilisée avec collecte des données par questionnaires autoadministrés. Cinq personnes ayant complété la formation sur deux jours, par vidéoconférence, ont pris part à l’étude. Les résultats montrent que les éléments de la formation ont été mis en œuvre avec un niveau élevé de fidélité relativement au modèle théorique. Les participantes mentionnent que la formation leur a permis de se sentir prêtes et capables d’offrir du soutien comme pairs aidants. Malgré le nombre limité de participants, cette étude suggère que la formation développée possède une valeur pratique pour la formation initiale des pairs aidants en santé mentale jeunesse. Elle révèle également que le cadre théorique utilisé est pertinent pour la formation de ces intervenants.