Fusarium proliferatum has been identified as the main causal agent of bulb rot of garlic (Allium sativum L.). This disease occurs after the drying process and can rot almost 30 % of the bulbs. Few studies are available regarding the effectiveness of chemical treatments to reduce F. proliferatum incidence in garlic. The efficacy of three commercial fungicides of different chemical groups to reduce seven strains of F. proliferatum mycelial growth was tested in vitro. These three fungicides were also evaluated by foliar spreading of aqueous suspension in a field crop. Fluopyram 20 % + tebuconazole 20 % and tebuconazole 50 % + trifloxystrobin 50 % were highly effective at reducing mycelial growth in F. proliferatum with EC 50 values <2 ppm. In general, the effectiveness of the fungicides was enhanced with increasing dosage. Our results indicate that the fungicides evaluated in this study may lead to a risk of resistance appearing in F. proliferatum at low concentrations and this risk is maintained at higher doses for the fungicide dimethomorph 7.2 % + pyraclostrobin 4 %. Although several of the fungicides affected in vitro mycelial growth

Diseases that affect garlic during storage can lead to severe economic losses for farmers worldwide. One causal agent of clove rot is Fusarium proliferatum. Here, the progress of clove rot caused by F. proliferatum and its dependence on different storage conditions and cultivar type were studied. The effect of temperature on mycelial growth, conidial viability, and fungal survival during garlic commercial storage was documented. Samples of 50 bulbs from a randomized field trial with three different clonal generations for purple garlic (F3, F4 and F5) and the F4 clonal generation for white garlic were labeled and stored for two months (short-term storage). In addition, another sample of the F5 clonal generation of purple garlic was stored for 6 months after harvest (long-term storage). The presence of the pathogen and the percentage of symptomatic cloves were evaluated. A notable difference in the rot severity index (RSI) of different garlic varieties was observed. In all studied cases, clove rot increased with storage time at 20 °C, and the white garlic variety had a higher index of rot severity after two months of storage. Additionally, there were clear differences between the growth rates of F. proliferatum isolates.Studies conducted on the temperature responses of the pathogen propagules showed that exposure for at least 20min at 50 °C was highly effective in significantly reducing the viability of fungal conidia.Pathogenicity studies showed that the fungus is pathogenic in all commercial varieties. However, there were significant differences in varietal susceptibility between Chinese and white garlic type cultivars (81.84 ± 16.44% and 87.5 ± 23.19% symptomatic cloves, respectively) and purple cultivars (49.06 ± 13.42% symptomatic cloves).

No abstract

During September 2011, post-emergence damping off of Swiss chard (Beta vulgaris subsp. cicla L.) was observed in a greenhouse in Villa del Prado (Spain). About 20% of the seedlings showed damping off symptoms. Lesions were initially water soaked, dark brown necrosis of crown tissue, irregular in shape and sunken in appearance on large plants, causing the infected seedlings to collapse and eventually die. Rhizoctonia solani was isolated consistently from symptomatic plants. After morphological and molecular identification of the isolates, pathogenicity was tested by placing agar plugs of four isolates adjacent to the stem at the three or four true leaf stage. In inoculated plants, brown crown and stem necrosis occurred while control plants did not show disease symptoms. Pathogenicity using non-germinated seeds was also tested. All four isolates produced extensive damping off when inoculated on non-germinated seeds. To our knowledge, this is the first report of damping off of Swiss chard caused by R. solani in Europe.Additional key words: Beta vulgaris subsp. cicla; damping-off; morphological identification; molecular identification; silver beet. ResumenComunicación corta. Rhizoctonia solani, agente causal de la muerte de plántulas de acelga en España En el mes de septiembre de 2011, se observó muerte de plántulas en acelgas cultivadas en un invernadero en Villa del Prado (España). Cerca del 20% de los semilleros mostraban síntomas de caída de plántulas. Las lesiones iniciales tenían un aspecto húmedo y consistían en una necrosis del tejido de la corona de color marrón oscuro, de bordes irregulares y deprimidos en las plantas adultas, produciendo el colapso de las plántulas y, ocasionalmente, la muerte de las mismas. Rhizoctonia solani se aisló de manera consistente de las plantas sintomáticas. Tras una identificación morfológica y molecular, la patogenicidad de los aislados se probó colocando discos de agar adyacentes al tallo de la planta en estados de tres o cuatro hojas verdaderas. En las plantas inoculadas se observó un oscurecimiento de la corona y la necrosis del tallo en todos los casos, síntomas que no mostraron las plantas control. Por otro lado, se comprobó la patogenicidad de los aislados en semillas no germinadas. Los cuatro aislados produjeron muerte de plántulas cuando se inocularon en semillas no germinadas. Hasta donde hemos podido comprobar, este trabajo parece ser la primera cita de muerte de plántulas de acelga causada por R. solani en Europa.