In a market undergoing constant evolution, the production of chicken meat that consumers would perceive as “natural” and “animal friendly” is crucial. The use of probiotics in rurally reared chickens could represent a major opportunity to achieve mutual benefit for both the industry and consumers. A total of 264 male Kabir chicks were randomly distributed to one of 2 dietary treatments: the L group received a commercial feed supplemented with 2.0 g/100 kg of Lactobacillus acidophilus D2/CSL, while the C group received the same basal diet without the additive. To assess the effects of probiotic supplementation in the chickens’ diet, productive performance was evaluated at d 21 and 42, whereas microbiological analyses of the intestinal content and intestinal histology and morphometry were performed at the end of the trial (d 42). At d 21 and 42, L birds showed better (P < 0.001) performance in terms of body weight, average daily gain, and feed conversion ratio. Enterococci, staphylococci, and Escherichia coli populations were not influenced by dietary treatment. On the contrary, Lactobacillus population increased (P = 0.032) in the L group. Furthermore, a tendency (P = 0.069) was observed for the coliforms to be influenced by diet, with lower values in the L group in comparison to the C group. Histological techniques revealed that the number of goblet cell containing neutral mucins was lower in the C group. Morphometric evaluations demonstrated that the probiotic supplementation increased the height of the mucosal layer by improving (P = 0.040) villus height, while crypt depth was unaffected. In conclusion, the results obtained in this study demonstrate that it is possible to use Lactobacillus acidophilus D2/CSL (CECT 4529) in rurally reared chicken breeds with positive effects on performance and gut health.

The development of specific actions to increase animal health and welfare is indicated as a strategy to improve the efficiency and sustainability of many livestock systems, including sheep farming. In this paper, efficiency measures are provided to confirm the hypothesis that farms that are higher-performing in terms of animal welfare and management are also more technically efficient. A two-stage Data Envelopment Analysis (DEA) approach was adopted with the following twofold objectives: 1) to evaluate the efficiency and super-efficiency of 76 meatproducing sheep farms situated in marginal lands in central Italy, through DEA and Super-DEA (S-DEA) models; and 2) to assess the influence of animal welfare and management indicators on technical efficiency values through the application of a Tobit regression model. An overall efficiency performance varying within a range of 0.44-1 was estimated, with an average value of 0.80, implying a potential increase of 20% in terms of output production from both management and scale improvements. The 'pure' technical inefficiency was found to contribute three times more than scale inefficiency in determining the overall technical inefficiency. Adopting a more extensive farming system and increasing replacement rate were found to affect negatively the efficiency scores. On the other hand, having less than 5% of animals with body condition score beyond acceptable limits, presence of access control structures, well managed lambing pens, and dedicated feed stocking areas resulted in a positive influence on efficiency. Improvements in animal welfare aspects did not appear to be farm-scale-dependent. HIGHLIGHTSDEA and Super-DEA models were applied to assess sheep farm technical efficiency in Central Italy. An overall potential 20% increase in output production was estimated. Animal welfare factors were found to significantly affect efficiency performance.