It is estimated that in developing countries 60% of waste is from lignocellulosic material that can be used in recycling processes. The purpose of this research is to find enzymatic cocktails from bacteria native to Ecuador that allow degradation of the lignocellulosic material, by searching native microorganisms collected in Los Andes, Amazonia and Antartida, which can be introduced in a process of bagasse enzymatic hydrolysis on an industrial scale. For this, qualitative and quantitative tests were carried out to measure the endoglucanase, exoglucanase and filter paper activity of the microorganisms and their enzymatic cocktails. Enzymatic hydrolysis tests were also performed on sugarcane bagasse. In addition, the value of the investments for the production of enzymatic cocktail of bacteria Bacillus sp. and the total cost of production was calculated. It was identified that Peniccillium sp. was the species with the highest activity of filter paper,

In the present work, enzymatic hydrolysis of sugarcane bagasse was carried out from a cocktail of enzymes produced by a bacterium native to Ecuador of the genus Bacillus sp., which is mixed with a commercial enzyme produced by Sigma. With this mixture 8 tests were performed changing 6 initial conditions. With the Plackett Bürman method, the factors that did not influence the process were discarded from the results of the glucose yield coefficients, thus leaving agitation speed and Tween 80 surfactant as the least significant factors in the levels studied. With this new adjusted model, the Box-Wilson complete factorial design of optimization of was used and it was concluded that the enzymatic cocktail of native and commercial enzymes generated glucose concentration results of 2.63 mg/ml and glucose yield of /100gMP. This is important because of the possible substitution of a percentage of commercial enzymes for native enzymes