Optimized multi-wall carbon nanotubes (MWNT) suspensions in aqueous solution have been obtained by joint use of ultrasonification and surfactant. A simple experimental procedure has been established to efficiently evaluate the dependence of the surfactant concentration on the MWNT concentration stable in suspension. The study of 3 different surfactants and MWNT provided by 3 suppliers showed that a threshold surfactant concentration exists above which the MWNT concentration is maximum. Furthermore, it is demonstrated that the maximum MWNT concentration achievable varies from 0.50 to 7.5 g/L depending mainly on quality of the MWNT determined by Raman spectroscopy analysis.