No abstract

This study was conducted to evaluate the salt tolerance ability of two soybean cultivars under salinity conditions using a selection of growth and physiological parameters. Three weeks after germination, plants began being treated with either a 100 mM concentration of NaCl in Hoagland solution or a non-salinity (0 mM NaCl) solution. The results showed that the plant height, number of leaves, leaf area index, and shoot and root biomasses were significantly reduced under salt conditions. A similar tendency was observed in several physiological parameters (SPAD, Fv/Fm). Since all the salt-treated plants of the D8 cultivar were dead after 40 days under the 100 mM NaCl treatment, the yield of D8 could not be obtained, while the treated plants of the D140 cultivar experienced a marked decrease in yield compared to the control plants. In this study, we identified that the D140 soybean cultivar had a better salt tolerance than the D8 soybean cultivar at a 100 mM NaCl concentration.

The C2H2 zinc finger protein ZAT12 has been classified as a plant core abiotic stress response gene in the early  response to multiple stresses. ZAT12 links the iron deficiency and oxidative stress responses through the direct interaction with/and negative regulation of a central regulator - FIT. For further research on the regulation of the ZAT12 protein in planta, a huge quantity of ZAT12 proteins is required to inject into mice for the generation of ZAT12 antiserum. In this study, the gene encoding the ZAT12 protein from Arabidopsis thaliana was cloned into the expression vector - pETBlue-2 and then overexpressed in E. coli T7. A high expression level was indicated by SDS-PAGE. Immunoblot demonstrated successful expression using a bacterial expression system.