Atmospheric CO2 impacts all aspects of plant development. It has changed in the past and is predicted to change further on. Studies on the response of crop plants to low and elevated CO2 concerning growth, productivity and physiological processes are intense. In contrast, the molecular mechanisms of cellular CO2 exchange are still under discussion. At the same time it becomes more and more accepted that carbon dioxide is transported across cellular biomembranes by CO2 conducting aquaporins. Our recent study (Boudichevskaia et al., 2015) demonstrates that the lack of a single gene product – aquaporin AtPIP1;2 – resulted in massive transcriptional reprogramming in Arabidopsis as a consequence of reduced tissue CO2 diffusion rates. Therefore, the transcriptome data of the aquaporin AtPIP1;2 deficient line can be used in the comparative expression analyses for better understanding the role of aquaporins with regard to CO2 and water transport in plants. Here we describe a gene expression dataset generated for three biological replicates per genotype on Affymetrix platform. We provide detailed methods and analysis on microarray data which has been deposited in Gene Expression Omnibus (GEO): GSE62167. Additionally, we provide the R code for data preprocessing and quality control.
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Abstract. The color red has been shown to affect psychological functioning. In performance settings, it is associated with negative emotions, avoidance motivation, and cognitive restriction. Because red is frequently used in written performance feedback, we examined whether it represents an external form aspect that can adversely influence the perception of critical feedback by the receiver, independent of the verbal content of the feedback. To this end, we conducted a web-based experiment in which 171 participants performed an alleged attention test and were then given moderately critical feedback, including hints for improvement. Per random assignment, either some of the words within the feedback were presented in red letters or all words were presented in standard black. The participants’ subsequent evaluation of the feedback revealed that using red in the feedback caused the feedback to be perceived as relatively less emotionally positive. There was no direct effect of using a red font in how the feedback was cognitively perceived (i.e., how helpful, fair, or comprehensible it was). However, preliminary evidence suggests that using a red font indirectly had an adverse effect on the cognitive feedback perception, mediated via the emotional feedback perception. As a practical implication, red should be used cautiously in critical feedback in order to avoid compromising the functions of feedback.
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