<p>supplementary figures 1 to 3: SF1 represents the aggregation of Colorectal cancer cells; SF2 concerns the expression of Cadherin 6 by colorectal cancer cell lines. SF3 depicts the involvement of Cadherin 6 in the interaction between platelets and colorectal cancer cells in static conditions. SF4 is a diagram summarizing the results described in this paper.</p>

<p>Supplemental methods concerning the antibodies and reagents used, the preparation of platelets, the purification of monocytes, the knock-down of Cdh6, the flow cytometry experiments, the immunohistochemistry, the measure of the TF and TFPI activity, the aggregation of platelets, the RT-PCR, the western-blotting, the gene expression analysis and the methods used to detect macrophages and performed the experimental metastasis model. This file contains also the legend for the supplemental Fig 1 to 4.</p>

<p>supplementary figures 1 to 3: SF1 represents the aggregation of Colorectal cancer cells; SF2 concerns the expression of Cadherin 6 by colorectal cancer cell lines. SF3 depicts the involvement of Cadherin 6 in the interaction between platelets and colorectal cancer cells in static conditions. SF4 is a diagram summarizing the results described in this paper.</p>

<p>Supplemental methods concerning the antibodies and reagents used, the preparation of platelets, the purification of monocytes, the knock-down of Cdh6, the flow cytometry experiments, the immunohistochemistry, the measure of the TF and TFPI activity, the aggregation of platelets, the RT-PCR, the western-blotting, the gene expression analysis and the methods used to detect macrophages and performed the experimental metastasis model. This file contains also the legend for the supplemental Fig 1 to 4.</p>

<div>Abstract<p>Platelets promote metastasis, however, their role in tumor growth remains controversial. Here, we investigated the effect of platelet interactions with colorectal tumor cells. Platelets extravasated into the tumor microenvironment and interacted with tumor cells in a cadherin-6–dependent manner. The interaction induced platelet spreading, release of their granule content, and the generation of three types of microparticles (iMP) that expressed platelet markers, tumor markers, or both. The presence of iMPs was confirmed in colorectal cancer tissue specimens. Platelets significantly reduced tumor growth and increased intratumoral macrophages. This was mediated by iMP recruitment of macrophages via the chemoattractants RANTES, MIF, CCL2, and CXCL12 and activation of their tumor cell killing capacity through IFNγ and IL4, which led to cell-cycle arrest of tumor cells in a p21-dependent manner. In contrast, in the bloodstream, iMPs activated endothelial cells and platelets and induced epithelial-to-mesenchymal transition of tumor cells, promoting metastasis. Altogether, these results indicate that depending on the environment, local or bloodstream, the consequences of the interactions between platelets and a tumor may promote or prevent cancer progression.</p>Significance:<p>Tumor cell interaction with platelets produces chimeric extracellular vesicles that suppress primary tumor growth by activating tumor-eliminating macrophages, while promoting metastasis through EMT and endothelial activation.</p></div>