Disk diffusion tests, inoculated directly from positive blood cultures, were evaluated for accuracy of reading zone diameters after 4-and 6-h and overnight incubation. In comparisons with results from standard disk diffusion tests, the 4-h results were in agreement for 83% of tests with gram-positive organisms and 64% of tests with gram-negative organisms. When minor discrepancies were ignored, the 4-h readings were in agreement for 98% of the tests with gram-positive organisms and 95% of the tests with gram-negative organisms. After 6 h of incubation, 91% of the tests with gram-positive organisms and 86% of the tests with gram-negative organisms agreed with standard results. The agreement was 99% for tests with both grampositive and gram-negative organisms when minor discrepancies were excluded. Very major discrepancies occurred in two tests (0.1 %) with gram-positive organisms and were not observed in tests with gram-negative organisms. The frequencies of major discrepancies were 3.5% after 4 h, 0.6% after 6 h, and 0.7% after overnight incubation. Ampicillin and cephalothin tests with Escherichia coli and Klebsiella spp. accounted for 81 % of the major discrepancies in tests with gram-negative organisms. Oxacillin tests accounted for more than half of the major discrepancies in tests with staphylococci. The results of this study, which did not include the newer antibiotics, indicate that direct susceptibility tests from blood cultures read after 6 h of incubation are more reliable than 4-h results and produce less than 1 % major errors in comparisons with standard susceptibility tests.

Seventy-five patients attending a clinic for chronic prostatitis were evaluated by use of lower urinary tract localization cultures. Coagulase-negative staphylococci, alpha-hemolytic streptococci, and diphtheroids were the most common isolates, but none of these organisms were pathogens, based on the absence of bacteriuria or evidence of an inflammatory response in prostatic secretions. Recognized uropathogens were isolated in 12 (16%) of the 75 cases and included Escherichia coli in 6 cases, Enterococcus spp. in 2 cases, and Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter cloacae, and Staphylococcus saprophyticus in 1 case each. Laboratory evaluation of men with chronic prostatitis should concentrate on the isolation and antimicrobial susceptibility testing of bacteria that have an established pathogenic potential in the genitourinary tract.

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A space-saving media table designed to facilitate pouring large quantities of plated media in a small area is described.

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