Bacillus amyloliquefancies CH51 isolated from cheonggukjang, a traditional Korean fermented soy food, has strong fibrinolytic activity and produces several fibrinolytic enzymes. Among four different growth media, tryptic soy broth was the best in terms of supporting cell growth and fibrinolytic activity of this strain. A protein with fibrinolytic activity was partially purified from the culture supernatant by CM-Sephadex and Phenyl Sepharose column chromatographies. Tandem mass spectrometric analysis showed that this protein is a homolog of AprE from B. subtilis and it was accordingly named AprE51. The optimum pH and temperature for partially purified AprE51 activity were 6.0 and 45 o C, respectively. A gene encoding AprE51, aprE51, was cloned from B. amyloliquefaciens CH51 genomic DNA. The aprE51 gene was overexpressed in heterologous B. subtilis strains deficient in fibrinolytic activity using an E. coli-Bacillus shuttle vector, pHY300PLK.
This study analysed trends of first-time patients visiting the paediatric psychiatry clinic in a university hospital. The medical records from 2009 to 2016 of first-time patients visiting the Kyung Hee university hospital were reviewed, focusing on children in grades 1–12. We analysed the prevalence rate of psychiatric disorders per 100,000 general populations by gender and grade, and the characteristics of patients who sought outpatient care more than three times. The study included 1,467 participants, of which 931 were males (63.5%). The number of male patients per 100,000 populations significantly decreased from 4.14 in 2009 to 2.03 in 2016. While hyperkinetic disorders had the highest prevalence in males, neurotic disorders were most frequent in females. Prevalence of disruptive behaviour disorders in males and mental retardations in females decreased significantly during the study period. The factors affecting continuity were being female, studying in grades 7–12, and diagnosis of depressive, hyperkinetic, and tic disorders. Physicians should consider the new paediatric patients’ gender, grade, and expected diagnosis from their first visit to improve treatment compliance.
That the schizophrenic is "out of contact with reality," and hence impervious to it, has been recognized from the time that the term "schizophreniz" was coined. That the members of the schizophrenic's family are also "impervious" (Lidz et al., 1958), and hence also "out of contact with reality," has only comparatively recently been recognized (Bateson et al., 1956; Wynne et al., 1958; Laing and Esterson, 1964). Imperviousness is here defined as an individual's inability (or unwillingness) to consciously grasp. become aware of, register, and acknowledge another person's interpersonal perception despite the fact that this perception has been clearly stated or presented to him.-1 This paper will focus on the levels of imperviousness that are characteristically established within the parent-child sub-system in the schizophrenix's family. This is not meant to deny the presence of imperviousness in the other subsystems within the family - i.e., between husband and wife or between the children themselves - for they, too, exist and are significant. However, to simplify matters, only imperviousness in the parent-child sub-system will be focused on here.
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