In a past study of hyperoxia-induced lung injury, the extensive lymphatic filling could have resulted from lymphatic proliferation or simple lymphatic recruitment. This study sought to determine whether brief lung injury could produce similar changes, to show which lymphatic compartments fill with edema, and to compare their three-dimensional structure. Tracheostomized rats were ventilated at high tidal volume (12-16 ml) or low tidal volume (3-5 ml) or allowed to breathe spontaneously for 25 min. Light microscopy showed more perivascular, interlobular septal, and alveolar edema in the animals ventilated at high tidal volume (P < 0.0001). Scanning electron microscopy of lymphatic casts showed extensive filling of the perivascular lymphatics in the group ventilated at high tidal volume (P < 0.01), but lymphatic filling was greater in the nonventilated group than in the group that was ventilated at low tidal volume (P < 0.01). The three-dimensional structures of the cast interlobular and perivascular lymphatics were similar. There was little filling and no difference in pleural lymphatic casts among the three groups. More edema accumulated in the surrounding lymphatics of larger blood vessels than smaller blood vessels. Brief high-tidal-volume lung injury caused pulmonary edema similar to that caused by chronic hyperoxic lung injury, except it was largely restricted to perivascular and septal lymphatics and prelymphatic spaces.

Background: Although formalin preserved organs in dissection-hall are used for teaching purpose but the odour and carcinogenic effect of formalin has made us to search for some alternate method for preparing teaching models. One of such methods is plastination but it requires well equipped laboratory and costly chemicals. So our objective was to develop a cheap and easy and feasible method of preparing formalin free teaching models of organs.

Infection of pilo-sebacious gland or traumatic migration of epidermis to the deeper layers of the skin can lead to epidermoid cyst. Any site of the body which is lined by squamous epithelium can be the site of epidermoid cyst. We present a case of sublingual epidermoid cyst in a 14-year-old female, who presented with a slow-growing, soft, midline swelling in submental region. Cyst was excised under local anesthesia with sedation. Histopathological examination revealed a cystic wall lined by keratinizing squamous epithelium with lamellated keratin and fibrocollagenous tissue with congested blood vessels, along with subcutaneous fat and muscle bundles, which is suggestive of epidermoid cyst.