Ambulatory arterial stiffness index (AASI) is a novel estimate of arterial stiffness which independently predicts cardiovascular mortality, even in normotensive subjects. Additionally, other markers derived from ambulatory blood pressure (BP) monitoring, including variability (BPV), pulse pressure (PP), nocturnal dipping, and morning BP surge have all been shown to be predictive of endorgan damage and cardiovascular disease. Exaggerated cardiovascular reactivity to sympathoexcitatory stimuli may also predict future incidence of hypertension. The purpose of this investigation was to test the hypothesis that AASI and other derivations of ambulatory BP, including PP, 24-hr BPV, dipping, and morning surge, would be correlated with the pressor response to common physiological stress maneuvers. We measured continuous HR and arterial BP during headup tilt, mental stress, cold pressor test, and isometric handgrip to fatigue in 67 healthy, normotensive, non-obese individuals (43 women, 24 men, mean age ± SD: 28 ± 6 yr). Then 24-hr ambulatory BP was obtained, and AASI was defined as 1 minus the slope of diastolic on systolic BP in individual 24-h ambulatory BP recordings. Although all measures were widely variable among subjects, there was no relationship between AASI, PP, BPV, dipping, and morning surge with the pressor responses. We conclude that in the absence of aging, cardiovascular, or autonomic disease, the novel stiffness index (AASI) or other ambulatory BP indices are either poorly correlated with, or mechanistically unrelated to the complex pressor response to common provocations of sympathoexcitation.