Introduction: Tobacco consumption alters many biological parameters, as well as α-amylase activity. This research work aims to study the activity of serum and salivary alpha-amylase in habitual adult tobacco consumers. Methods:We carried out a cross-sectional descriptive and analytical study on 234 adults (54 smokers, 60 snuffers, 60 chewers and 60 tobacco non-consumers). Serum and salivary alpha-amylase was measured using kinetic enzymatic method. ANOVA, and Kruskal-Wallis test were used to compare averages according to cases. Linear regression enabled to establish relationships between duration of consumption, quantity of tobacco consumption, as well as serum and salivary α-amylase activity. Results and conclusion:The mean activity of serum (UI/L) and salivary (10 4 UI/L) alpha-amylase was respectively 110.