Lake Tanganyika is the oldest and deepest African Great Lake and harbours one of the most diverse fish assemblages on earth. Two clupeid fishes, Limnothrissa miodon and Stolothrissa tanganicae, constitute a major part of the total fish catch, making them indispensable for local food security. Parasites have been proposed as indicators of stock structure in highly mobile pelagic hosts. We examined the monogeneans Kapentagyrus limnotrissae and K. tanganicanus (Dactylogyridae) infecting these clupeids to explore the parasites' lake-wide population structure and patterns of demographic history.Samples were collected at seven sites distributed across three subbasins of the lake. Intraspecific morphological variation of the monogeneans (N = 380) was analysed using morphometrics and geomorphometrics of sclerotised structures. Genetic population structure of both parasite species (N = 246) was assessed based on a 415 bp fragment of the mitochondrial COI gene.Overall, we observed a lack of clear geographical morphological differentiation in both parasites along a north-south axis. This lack of geographical population structure was also reflected by a large proportion of shared haplotypes, and a pattern of seemingly unrestricted gene flow between populations. Significant morphological and genetic differentiation between some populations might reflect temporal differentiation rather than pure geographical isolation. Overall, the shallow population structure of both species of Kapentagyrus reflects the near-panmictic population structure of both host species reported in previous studies. Morphological differences related to host species identity of K. tanganicanus were consistent with incipient speciation observed at the genetic level. Both parasite species experienced a recent demographic expansion, which might be linked to paleohydrological events. Finally, hybridisation between species of Kapentagyrus was found, representing the first case in dactylogyrid monogeneans.

Democratic Republic of the Congo. 13. Appui à la Lutte contre l'Oppression faite à l'Enfant et à la femme (ALOE), Uvira, Democratic Republic of the Congo. 14. Institut Supérieur des Techniques Médicales (ISTM) de Bukavu, Bukavu, Democratic Republic of the Congo.

Knowledge on the reproductive biology of the endemic clupeids Limnothrissa miodon and Stolothrissa tanganicae, two main target species of the pelagic fisheries of Lake Tanganyika, is constrained by fragmented monitoring activities. Here, we investigate the nursing areas of L. miodon, the timing of reproductive activities of littoral and pelagic L. miodon, and the timing of reproductive activities of pelagic S. tanganicae in the Congolese waters of the northern end of Lake Tanganyika (Bujumbura sub-basin). Nursing areas were determined year-round (2009–2010) based on the presence of clupeid larvae at two sandy and two stony beaches. The gonadosomatic index (GSI) and the proportion of fish having ripe gonads were used to study variation in reproductive indices in space (littoral vs. pelagic zones) during one year (2013–2014), as well as in time (dry vs. rainy season) during three years (2013–2016). Larvae of L. miodon were more frequently encountered on sandy than on stony beaches. Mature L. miodon females were more abundant in the littoral than in the pelagic zone, while the proportion of mature males in both habitats was similar. Irregular, low amplitude peaks could be distinguished in the GSI and proportion of mature males and females, but averages only differed between the dry and the rainy season in males. In contrast, GSI and proportions of mature males and females in S. tanganicae were higher in the dry season than in the rainy season. The reproductive effort of males and females of S. tanganicae and littoral L. miodon, but not pelagic L. miodon, was strongly synchronized. Interestingly, reproductive investment was also synchronised between pelagic male L. miodon, and pelagic S. tanganicae. Our time series strongly supports the view that L. miodon reproduces year-round in the littoral zone, while reproduction in S. tanganicae is seasonal. For fisheries management, we recommend year-round protection of sandy beaches, which are the main breeding grounds for L. miodon.

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Background The hydrogeological history of Lake Tanganyika paints a complex image of several colonization and adaptive radiation events. The initial basin was formed around 9–12 million years ago (MYA) from the predecessor of the Malagarasi–Congo River and only 5–6 MYA, its sub-basins fused to produce the clear, deep waters of today. Next to the well-known radiations of cichlid fishes, the lake also harbours a modest clade of only two clupeid species, Stolothrissatanganicae and Limnothrissamiodon. They are members of Pellonulini, a tribe of clupeid fishes that mostly occur in freshwater and that colonized West and Central-Africa during a period of high sea levels during the Cenozoic. There is no consensus on the phylogenetic relationships between members of Pellonulini and the timing of the colonization of Lake Tanganyika by clupeids. Results We use short-read next generation sequencing of 10X Chromium libraries to sequence and assemble the full mitochondrial genomes of S.tanganicae and L.miodon. We then use Maximum likelihood and Bayesian inference to place them into the phylogeny of Pellonulini and other clupeiforms, taking advantage of all available full mitochondrial clupeiform genomes. We identify Potamothrissaobtusirostris as the closest living relative of the Tanganyika sardines and confirm paraphyly for Microthrissa. We estimate the divergence of the Tanganyika sardines around 3.64 MYA [95% CI: 0.99, 6.29], and from P.obtusirostris around 10.92 MYA [95% CI: 6.37–15.48]. Conclusions These estimates imply that the ancestor of the Tanganyika sardines diverged from a riverine ancestor and entered the proto-lake Tanganyika around the time of its formation from the Malagarasi–Congo River, and diverged into the two extant species at the onset of deep clearwater conditions. Our results prompt a more thorough examination of the relationships within Pellonulini, and the new mitochondrial genomes provide an important resource for the future study of this tribe, e.g. as a reference for species identification, genetic diversity, and macroevolutionary studies.