Dyckia vicentensis is an endemic species of the southwestern region of Rio Grande do Sul (RS, Brazil), which presents high ornamental and economic value. Thus, the aim of this study was to test in vitro and ex vitro conditions for its propagation. For in vitro germination, disinfested seeds were inoculated on Murashige and Skoog medium with different salt concentrations and containing or not active charcoal. The ex vitro emergence of the seeds was evaluated using different compositions of substrates. Results showed that the propagation of D. vicentensis could be successfully performed in vitro on medium with 50% salt concentration or ex vitro using commercial substrate. The seedlings showed good adaptation during acclimatization in a greenhouse, although the in vitro germinated plants presented higher survival rate, number of leaves, and biomass in relation to those grown in ex vitro substrate. This is the first study carried out on the propagation of D. vicentensis, which may be used to subsidize its propagation and conservation.

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