Virtual learning environments are commonly adopted by universitiesas classroom teaching support. One of the resources they offerare class presentations, usually in PDF files. Considering that webaccessibility is essential to allow users with some limitations tounderstand, interact, operate and navigate web content, this articlepresents an assessment of the accessibility of PDF documents madeavailable on Moodle. The methodology used a hybrid approachcomprised techniques and practices for assessing human-computerinteraction and assessing accessibility with users simulating blindness.A quantitative analysis of the data was carried out consideringthe accessibility problems faced by users in elements such as tables,graphs, mathematical formulas, expressions in foreign languagesand presentation topics. The results indicated accessibility problemsin all documents in the sample, even causing users to drop outduring the evaluations.