Essential oils can have an impact on behavioural responses of cigarette beetle (Lasioderma serricorne). In this study, we provide evidence that some chemical compounds in essential oil of tobacco (Nicotiana tabacum) leaves, which were subjected to hydrodistillation and extracted with n-hexane, can strongly attract L. serricorne significantly. Two main chemical constituents, 6, 8-nonadien-2-one, 8-ethyl-5 -(1-methylethyl) and 1-(2, 6, 6-trimethyl-1, 3-cyclohexadien-1-yl) 2 -buten-1-one, in the essential oils were confirmed by GC-MS analysis. This result could be helpful to find some chemical compositions from N. tabacum as leading compounds for development of new bionic attractant.