Coating of various synthetic, absorbable, and biologic meshes with mesenchymal stem cells (MSCs) and fibroblasts was analyzed qualitatively and quantitatively. Five hernia meshes-light weight monofilament polypropylene (Soft Mesh), polyester (Parietex-TET), polylactide composite (TIGR), heavy weight monofilament polypropylene (Marlex), and porcine dermal collagen (Strattice)-were coated with three cell lines: human dermal fibroblasts (HFs), rat kidney fibroblasts (NRKs), and rat MSCs. Cell densities were determined at different time points. Samples also underwent histology and transmission electron microscopic (TEM) analyses. It required HFs 3 weeks to cover the entire mesh, while only 2 weeks for NRKs and MSCs to do so. MSCs had no preference for any of the meshes and produced the highest cell densities on Parietex and TIGR. Substrate-preference accounted for the significantly lower fibroblast densities on TIGR than Parietex. Fibroblasts failed to coat Marlex. Strattice, which had the least surface area, generated comparable cell densities to Parietex. Both histology and TEM confirmed cell coating of mesh surface. Various prosthetics can be coated by certain cell strains. Both mesh composition and cell preference dramatically influence the coating process. This methodology provides foundation for novel avenues of modulation of host response to various modern synthetic and biologic meshes.

No abstract

According to the principle of the experimental modal analysis, this study is based on tool system of the MIKRON UCP 710 numerical control machining center as test object for experimental modal analysis. Using the integral polynomial recognition method to identify the modal parameters (natural frequency, structural damping, and modal shape), and finally matching the results with the vector analysis method and the finite element simulation method. The results show that integral polynomial recognition method has higher precision than the vector analysis method to identify the multi-degree of freedom system; the experimental modal analysis can also obtain better modal parameters of the structure system, and a higher precision than the finite element simulation method. Obtained the MIKRON UCP 710 high-speed milling center tool system accurate modal parameters provides the necessary theoretical and experimental basis for the further study of the stability properties in the cutting processing of the high speed machining tool system.