Chemiluminescence immunoassay (CLIA) offers several potential advantages and has applications in clinical chemistry, bioanalysis and environmental analysis. This review covers recent developments and applications of CLIA in immunorecognition, CL markers and related techniques, and solid-phase materials. We describe automation, integration and miniaturization of CLIA. We evaluate different CLIA systems and give some pointers as to the likely directions of future developments. ª

We demonstrate the inscription of fiber Bragg gratings by 193 nm ArF excimer laser in microfibers drawn from the standard single mode telecommunication fiber. Fiber Bragg gratings are directly inscribed in a series of microfibers with diameter ranged from tens of μm to 3.3 μm without hydrogen loading or other treatment to photosensitize the microfibers. Four reflection peaks are observed where three correspond to high order mode resonances. The resonance wavelength depends on the fiber diameter and it sharply blueshifts as the diameter is decreased below 10 μm. The gratings are characterized for their response to ambient refractive index. The higher order mode resonance exhibits higher sensitivity to refractive index.

We demonstrate an ultrasensitive refractive-index (RI) sensor utilizing the polarimetric interference of a rectangular silica microfiber. The measured sensitivity is as high as 18,987 nm/RIU (refractive-index unit) around the RI of 1.33, which is 1 order of magnitude higher than that of the previously reported microfiber devices. Theoretical analysis reveals that such high sensitivity not only is originated from the RI-induced birefringence variation but also relies on the unique birefringence dispersion property for the rectangular microfiber. We predict that the sensitivity can be enhanced significantly when the group birefringence approaches zero.

Nucleic acid detection with label-free biosensors circumvents the need for costly fluorophore functionalization steps associated with conventional assays by utilizing optical fiber transducers. In spite of their technological prowess, however, these biosensors' sensitivity is limited by the design/configuration of their transducers. Therefore, it is imperative to integrate novel optical fiber transducers with existing label-free approaches to overcome those limitations. Herein, we present a high sensitivity label-free fiber optic biosensor that employs polarimetric interference of a high-birefringence (Hi-Bi) microfiber to specifically detect DNA molecules. A slight target DNA concentration change is converted into an optical wavelength shift of polarimetric interference generated by the microfiber Sagnac interferometer. The sensor provides a log-linear response to target ssDNA concentrations range from 100 pM to 1 μM and a minimum detectable concentration of 75 pM.

A Mach-Zehnder interferometer (MZI) composed by a pair of long period gratings (LPGs) fabricated in silica microfiber for sensing applications is demonstrated. Each LPG is fabricated with a pulsed CO2 laser by creating six periodical deformations along fiber length with only one scanning cycle. The length of the MZI can reach as short as 8.84 mm when the diameter of the microfiber is 9.5 μm. Compared with the ones fabricated in single-mode fibers, the present MZI is much shorter owing to the large effective-index difference between the fundamental and higher order modes. The microfiber MZI exhibits a sensitivity to surrounding refractive index (RI) of 2225 nm per refractive index unit and the temperature sensitivity of only 11.7 pm/°C. Theoretical analysis suggests that the performances of the MZI sensor can be improved by using thinner microfibers with a diameter down to 3.5 μm: The sensitivity can be greatly enhanced due to the stronger evanescent-field interaction and reduced dispersion factor; the transmission dips become narrower which benefits high-resolution measurement; the thinner fiber also allows further reduction in device length. The present device has great potential in biochemical and medical sensing due to the advantages including easy fabrication, excellent compactness and high sensitivity.