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Background: Monoclonal antibody F77 raised against the PC-3 cells recognizes human prostate cancers.Results: Co-transfections with glycosyltransferase genes showed that FUT1 and one of GCNT1, GCNT2, or GCNT3 are necessary for F77 antigen expression in mammalian cells.Conclusion: F77 antigen is expressed on glycan structures composed of Fucα1→2Galβ1→4GlcNAcβ1→6Gal/GalNAc.Significance: Defining the F77 epitope provides a basis for clinical applications of this antibody.

The neoglycolipid (NGL) technology is the basis of a state-of-the-art oligosaccharide microarray system, which we offer for screening analyses to the broad scientific community. We review here the sequential development of the technology and its power in pinpointing and isolating naturally occurring ligands for glycan-binding proteins (GBPs) within glycan populations. We highlight our Designer Array approach and Beam Search Array approach for generating natural glycome arrays to identify novel ligands of biological relevance. These two microarray approaches have been applied for assignments of ligands or antigens on glucan polysaccharides for effector proteins of the immune system (Dectin-1, DC-SIGN and DC-SIGNR) and carbohydrate-binding modules (CBMs) on bacterial hydrolases. We also discuss here the more recent applications to elucidate the structure of a prostate cancer- associated antigen F77 and identify ligands for adhesins of two rotaviruses, P[10] and P[19], expressed on an epithelial mucin glycoprotein.

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