<p><em>Tea (</em>Camellia sinensis<em> (L.) O. Kuntze) is a cross-pollinated plant that has self-incompatible character. Assembly of superior clones through artificial pollination requires information of genetic relationships between accessions as a reference for parental lines selection. The study was aimed to determine the genetic diversity and relationships of 49 tea clones based on leaf morphology and yield components. The research was conducted at Pasir Sarongge experimental garden, Cianjur, West Java, from April to November 2015. The observed morphological characters were leaf length and width, leaf area, leaf angle, number of vein leaf, and internode between first and second leaves. Meanwhile, yield components were pecco number, pecco weight (p+3), banji bud number, banji bud weigth (b+1), and yield. The data were then used for descriptive analysis and grouping using UPGMA method based on dissimilarity matrix by XL-STAT software version 2009. The research showed that 49 clones observed here have variability on yield, leaf area, number of banji bud, and pecco number with coefficient of diversity 27.77%–51.83%. On the other hand, result of cluster analysis divided tea clones into four groups. The first group consisted of 34 clones with morphological characteristics similar to sinensis type (narrow leaves and low productivity). Group II comprised 12 clones with morphological characteristics (wide leaf) and productivity (high) similar to assamica type. Group III and IV, each contained one clone, and have similarity to assamica. The most far genetic relationships was found between group I and II (55.59%), while the closest one observed between group III and IV (5.76%).</em></p>

Effectiveness of BPMC insecticide and suren leaf extract on brown planthopper (Nilaparvata lugens Stal.) and its natural enemy population on Ciherang rice varietyEffectiveness study of BPMC insecticide and suren leaf extract esticide against brown planthopper and its side-effect on its natural enemy population on paddy Ciherang variety was conducted in rice field of Balai Besar Peramalan Organisme Pengganggu Tumbuhan (BBPOPT), Karawang, West Java from May to August 2015. The experimental design used was Randomized Blok Design with 7 treatments and 4 replications ie suren leaf extract of 50 g/l, 100 g/l and 150 g/l, three concentrations of BPMC of 0.5 ml/l, 1.0 ml/l and 1.5 ml/l, and a control treatment. The results showed that BPMC insecticide and suren leaf extract were able to suppress the population of brown planthopper and influenced its natural enemy population. The treatment of 50 g/l suren leaf extract gave the highest suppression of the brown planthopper population. It was also caused no negative impact to the natural enemies of P. fuscipes and L. pseudoannulata. The application of 1.5 ml/l of BPMC insecticide gave the highest grain weight average of 37.80 kg/plot compared to the lowest production in control treatment of 32.63 kg/plot. Therefore, application of BPMC insecticide and suren leaf extract effectively suppressed the brown planthopper population and the natural enemy existence was assumed to affect the rice grain production in this study.Keywords: BPMC insecticide, Natural enemy, Suren, PlanthopperABSTRAKStudi keefektifan insektisida BPMC dan ekstrak daun suren terhadap hama wereng batang coklat (WBC) dan efek samping terhadap musuh alami wereng pada padi varietas Ciherang dilaksanakan di sawah Balai Besar Peramalan Organisme Pengganggu Tumbuhan (BBPOPT) Karawang mulai Mei 2015 sampai Agustus 2015. Rancangan yang digunakan Rancangan Acak Kelompok (RAK) dengan 7 perlakuan dan 4 ulangan yaitu perlakuan tanpa insektisida (kontrol), 50 g/l ekstrak daun suren, 100 g/l ekstrak daun suren, 150 g/l ekstrak daun suren, 0,5 ml/l insektisida BPMC, 1,0 ml/l insektisida BPMC, dan 1,5 ml/l insektisida BPMC. Hasil menunjukkan perlakuan insektisida BPMC dan ekstrak daun suren dapat menekan populasi hama WBC dan memengaruhi populasi musuh alaminya (Paederus fuscipes dan Lycosa pseudoannulata). Perlakuan ekstrak daun suren konsentrasi 50 g/l dapat menekan populasi WBC tertinggi. Aplikasi ekstrak daun suren tidak berdampak negatif terhadap musuh alami P. fuscipes dan L. pseudoannulata. Perlakuan 1,5 ml/l insektisida BPMC menghasilkan produksi gabah tertinggi dari semua perlakuan dengan rata-rata bobot basah gabah 37,80 kg/petak dibandingkan dengan hasil terendah pada perlakuan kontrol sebesar 32,63 kg/petak. Dengan demikian, aplikasi insektisida BPMC dan ekstrak daun suren berpengaruh terhadap populasi hama WBC dan efek samping dari tingkat keberadaan musuh alami berakibat pada hasil gabah tanaman padi.Kata Kunci: Insektisida BPMC, Musuh alami, Suren, Wereng

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Torch ginger (Etlingera spp.) is one of the herbal plants native to Indonesia that has long been known and used as medicines. Exploration on torch ginger plant needs to be done to conservate and determine genetic diversity. Study of genetic diversity torch ginger in the district of Pangandaran is important because as long as there has been no study in depth the potential of genetic diversity and phylogenetic relationship germplasm of torch ginger Genetic diversity based on this research had a proportion of 47% of the total of 100% in the PC1-PC3 was based on the analysis Principal Component Analysis (PCA) with the character influencing that form clumps, line leaf midrib, plant height, stem diameter, leaf length, color scales rhizome, stem color, rhizome and the leaf base forms. Based on the results of the genetic diversity cluster analysis found that narrow torch ginger euclidean indicated by coefficient of 0.79 and was divided into six clusters. Accession which had the closest kinship i.e. accession N1 from Cikadu subdistrict of Cintakarya and accession N2 from Cikoang subdistrict of Cintakarya, while accession with the farthest kinship namely accession N29 from Jangraga subdistrict of Mangunjaya with range euclidean of 0.79.

Lili (Lilium sp.) termasuk famili Liliaceae, merupakan tanaman hias yang memiliki nilai ekonomi tinggi karena permintaan konsumen terus meningkat. Permintaan varietas tanaman yang seragam menuntut pengembangan hibrida F1. Perakitan tanaman hibrida dapat dihasilkan melalui pembentukan tanaman haploid. Salah satu metode untuk memproduksi tanaman haploid adalah dengan kultur antera.  Pengecekan tanaman hasil kultur antera dapat dilakukan dengan penghitungan  jumlah kloroplas dan jumlah kromosom, namun untuk lili hasil belum diperoleh informasi mengenai korelasi antara jumlah kloroplas pada sel penjaga stomata dengan jumlah kromosom, sehingga tujuan penelitian ini adalah untuk mengatahui korelasi antara jumlah kloroplas dengan jumlah kromosom serta mengetahui tingkat ploidi pada regeneran lili hasil kultur antera. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Hias (BALITHI) Cianjur dari Januari-Juni 2016.  Terdapat 5 nomor lili yang diuji tingkat ploidinya.  Setiap nomor terdiri dari 4 ulangan, setiap ulangan ada 4 botol dan  masing-masing botol terdiri dari 3 planlet. Metode analisis yang digunakan yaitu statistik sederhana rata-rata dan standar eror dan dikorelasikan. Hasil penelitian menunjukkan planlet haploid terbanyak ditemukan pada nomor 2015.1.1 kelompok Longiflorum dengan jumlah 26,67% sedangkan planlet haploid yang jumlahnya paling sedikit ditemukan pada nomor 2015.S2.3 kelompok Oriental dengan jumlah 11,11%. Metode kultur antera dapat menghasilkan planlet haploid namun pada Lilium sp. persentase keberhasilannya masih rendah. Tidak diperoleh korelasi antara jumlah kloroplas dan kromosom. Penelitian lebih lanjut terkait tingkat ploidi tanaman lili dan pengujian jumlah kromosom dengan menggunakan flowcytometer perlu dilakukan.  Lily (Lilium sp.), Liliaceae family, is an ornamental plant that has a high economic value as consumer demand continues to rise. The uniformity of crop varieties requires the development of F1 hybrids that can be generated through the formation of haploid plants. A method for producing haploid plant is by anther culture. Evaluation of anther culture can be done by counting the number of chloroplasts and the number of chromosomes in the regenerants.  However, lilies yet obtained information on the correlation between the numbers of chloroplasts in stomatal guard cells with chromosome numbers. Therefore, the purpose of this research aimed to know the correlation between the number of chloroplasts and chromosomes and to determine ploidy level in the regenerants of lily from anther culture. This research was conducted at Tissue Culture Laboratory of Indonesian Ornamental Crops Research Institute (IOCRI), Cianjur from January to June 2016. There were 5 numbers of lilies regenerant from anther culture that evaluate for ploidy level test.  Each number has four replications, each replication contained four bottles, and each bottle has three plantlets. The statistical analysis used statistical descriptive with average, standard error and correlation. The results showed, haploid plantlets were observed in 2015.1.1 plantlets from Longiflorum group with 26,67% while least number of haploid plantlets is found in 2015.S2.3 plantlets from Oriental group (11,11%).  The method of anther culture is able to produce haploid plantlets but the success rate was low in Lilium sp. There were no correlation between the number of chloroplasts and chromosomes.  Further studies related to the ploidy level of lilies from anther culture and the evaluation of chromosomes number by using flow cytometry requires to develop haploid plant of Lily.