From year to year, practicing various sports by amateur athletes is becoming more and more popular. One of such sports is road cycling. To achieve very good sports performance athletes should pay attention not only to physical activity but also to proper nutrition and hydration of the body. The aim of the study was to assess amateur cyclists' dietary habits, especially nutrition and hydration, including assessment of the regularity of eating meals, type of consuming products and fluid intake preferences. The study recruited 41 men aged 23–75 years (43.76 ± 13.25) participating in amateur race road cycling. To obtain information about nutrition and hydration, an original questionnaire was used. Out of all participants, 65.9% declared that they pay attention to their diet, and as many as 75.6% indicated that they eat meals regularly. The vast majority (43.9%) of the cyclists declared consuming four meals a day. Most of the cyclists consumed meat products several times a week—73.2% and dairy products—43.9%. The participants most often chose only one portion of fruit (41.5%) and vegetables (31.7%) during the day. The vast majority of cyclists consumed 3 L of fluids on a training day—51.2%. It turned out that all of the athletes hydrated during training: before it was 68.3% and after training—92.7%. We conclude that the amateur cyclists pay a lot of attention to their nutrition and hydration. During the day, most athletes eat an appropriate amount of meals on a regular basis and drink the right amount of fluids. However, eating of dairy, fruits and vegetables, or hydrating before exercising is insufficient.