The compounds, derivatives of olive leaves have a high antioxidant activity. The content of the total phenolic compounds (TPC), antioxidant activity (AOA) and HPLC polyphenol profile of methanol extracts from the leaves of the olive cultivars Chondrolia Halkidiki, Kalamon, Koroneiki grown in the nursery (in vivo) and in vitro plants of Chondrolia Halkidiki were compared. The results obtained for TPC varied between 9.2±0.5 mgGAE*gDW -1 and 16.4±0.5 mgGAE*gDW -1 . Antioxidant capacity was determined by four methods DPPH, ABTS, FRAP and CUPRAC. The highest results for TPC and AOA were achieved for the leaves of Chondrolia Halkidiki grown in vitro. A high correlation between the results gained from the TPC and AOA was established. Conducted HPLC analysis revealed the presence of 3,4-dihydroxybenzoic, caffeic, sinapic and ferulic acids and quercetin, hesperidin and luteolin and the quercetin glycosides rutin and hyperoside.
The effect of the soil herbicide isoxaflutole on the growth and development of new corn hybrids in controlled conditions was studied. Conducted assays confirmed that tested hybrids exhibit different sensitivity to herbicide treatment, expressed as a decrease of chlorophyll or increase of anthocyanin content. The lowest content of chlorophylls was determined for hybrid P0216 and the highest anthocyanin levels were observed for hybrid P9241. Present investigation was performed for evaluation the toxic effect of the herbicide and demonstrated that plant pigment content can be used as a marker for abiotic stress. This study could be very useful for practical applications as a preliminary test for phytotoxicity and/or sensitivity of different crops or model plants to herbicides.
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