A phytochemical investigation of Physalis minima led to the isolation of six withanolides, including withanolide E (1), withaperuvin C (2), 4β-hydroxywithanolide E (3), 28-hydroxywithaperuvin C (4), physaperuvin G (5), and 4-deoxywithaperuvin (6). Their chemical structures were elucidated by 1D-NMR and 2D-NMR data, as well as comparison with the data reported in the literature. All isolated compounds were evaluated for their cytotoxic activity against HepG2, SK-LU-1, and MCF7 cancer cell lines. As the obtained results, compounds 1 and 3 displayed the strongest cytotoxicity against HepG2, SK-LU-1, and MCF7 cell lines with IC50 value ranging from 0.051 ± 0.004 to 0.86 ± 0.09 μg/mL.

Study on different characteristics of TanRai aluminum hydroxide dissolution process in acid and base media were carried out on unactivated and activated samples using 3000rpm centrifugal grinding method under other conditions. The experimental results showed that the centrifugal grinding process did not change the solid phase structure (Gibbsite), and the specific surface of the sample was increased by 1.5 times, average particle size was increased by 10 times, the surface morphology during process of TanRai aluminum hydroxide dissolution were different. In the case of the process of Al(OH)3 dissolution in NaOH media, the corrosion reaction occurs on the all surface of the particle. It goes deep and accesses into the particle. The specific surface might be increased by 10 times in reaction processing, so the grinding did not increase recovery efficiency. In case of dissolution Al(OH)3 in HCl media, the reaction occurs on surface particle so the grinding effects to dissolution recovery that increasing from 2 to 5 times.

Phương pháp xác định hàm lượng Crinamidin bằng sắc ký khí khối phổ hai lần (GC-MS/MS) đã được xây dựng và thẩm định trên nền mẫu thực phẩm chức năng. Phương pháp được thực hiện trên cột DB5-MS, kết hợp với detector khối phổ ba tứ cực. Phương pháp được đánh giá về độ đặc hiệu, đường chuẩn, độ đúng, độ chụm, giới hạn phát hiện (LOD) và giới hạn định lượng (LOQ). Đường chuẩn được xây dựng trong khoảng nồng độ từ 5ppm-50ppm với hệ số tương quan là 0,9970. Độ thu hồi với ba mức nồng độ đều đạt trên 90,1%. Giới hạn phát hiện và giới hạn định lượng của phương pháp lần lượt là 3 và 10 mg/kg. Tất cả các kết quả thu được đều đáp ứng yêu cầu của AOAC trên nền mẫu thực phẩm chức năng. Phương pháp được ứng dụng để phân tích 34 mẫu thực tế trên địa bàn Hà Nội.

The determination of crinamidine in dietary supplements content by mass spectrometry (GC-MS/MS) was developed and validated. The method was performed on the DB5MS column (30m x 0,25mm; 0,25 μm), in combination with the tandem mass spectrometry. The parameters of the method were evaluated for selectivity, calibration curve, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The calibration curve was highly linear (the coefficient of determination 0.9970) within the concentration range of 5ppm-50ppm. The recovery at three concentrations were above 90,1%. The limit of detection (LOD) and limit of quantification (LOQ) of the methods were 3 and 10 mg/kg, respectively. All obtained results were acceptable according to the AOAC for dietary supplements. The method was applied to analyze 34 samples in Hanoi.