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A headspace (HS)/solid phase microextraction (SPME)/gas chromatography-mass spectrometry (GC-MS) method was developed to determine the profiles of volatile compounds from three Cuban unifloral honeys: Turbina corymbosa (L.) Raf., Ipomoea triloba L. and Gouania polygama (Jacq.) Urb. The optimization of the extraction conditions was carried out by response surface methodology. The analytical conditions of the optimized HS-SPME method were: 65 mm PDMS/DVB fiber, 6 g honey, 3 mL water and 20% w/w sodium chloride, 20 min for pre-extraction, 30 min for extraction at 60C and 4 min for GC desorption. The GC-MS analysis allowed the identification of 181 volatile compounds in these honeys. Possible markers for the floral classification of honeys were for the T. corymbosa type: 4-methyl methoxysalicylate, trans-pinocarveol, chavicol, methyl anisate, trans-abergamotene and a-bisabolol. In I. triloba honey, 2,5,5-trimethyl-3-tetrahydrofuranone and 1,2-diphenylethanol were found, while 5 Corresponding

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Objective:Zinc (Zn) deficiency is the most common nutrient deficiency in HIV, and is not reversed with ART. Zn deficiency affects the immune system and may be aggravated by excessive alcohol use. We examined the effect of alcohol consumption on plasma and dietary Zn, and its association with disease progression in HIV+ adults.Methods:After consenting, the Alcohol Use Disorders Identification Test (AUDIT) score 蠅8 was used to identify excessive alcohol intake among 274 HIV+ adults. Nutrient intake was estimated with two 24‐hour dietary recalls; blood was drawn for plasma Zn levels, and parameters of HIV disease progression were obtained from medical charts.Results:Mean age was 45.9±7.8, 64% were male, 69% were Black, 43% had excessive alcohol drinking, 65% were Zn deficient, 40% used multivitamins with 15 mg Zn/day. CD4 cell count was lower in excessive alcohol drinkers compared to non‐drinkers or moderate drinkers (374.8±54.1 vs. 459.2±62.6 cells/mm3, P=0.029). CD4 cell count and viral load were better in non‐drinkers and moderate drinkers (β=0.2, SE=1.7, P=0.03 and β=‐0.18, SE=0.22, P=0.049, respectively) than in excessive drinkers. There was no relationship between excessive drinking and Zn deficiency. Excessive drinking was correlated with tobacco (r=0.24, P<0.001) and illicit drug use (r=0.25, P<0.001). Zn intake was correlated with energy (r=0.491, P<0.001) and protein (r=0.718, P<0.001) intake, but not with plasma levels of Zn.ConclusionModerate or no alcohol consumption is associated with better measures of HIV disease progression than excessive drinking, but is not associated with Zn deficiency.Funded by NIDA & NIAAA