A general and efficient oxyhalogenation of unsaturated ketoximes has been achieved through copper catalysis with diethyl bromomalonate, N-chlorosuccinimide, and N-iodosuccinimide, yielding 5-chloromethyl, 5-bromomethyl, and 5-iodomethyl isoxazolines in good to excellent yields.

Aims/Introduction Long non‐coding RNAs (lncRNAs) exert essential functions in the pathogenesis of diabetic nephropathy (DN). LncRNA T‐cell factor 7 (TCF7) and semaphorin‐3A (SEMA3A) have been found to be involved in the progression of diabetic nephropathy. However, whether the effect of TCF7 on the pathogenesis of diabetic nephropathy is mediated by SEMA3A remains unclear. Materials and Methods TCF7, miR‐16‐5p, and SEMA3A were quantified by a qRT‐PCR or immunoblotting method. A CCK‐8 assay gauged the cell viability. Measurement of cell apoptosis was done using flow cytometry. RNA immunoprecipitation (RIP), dual‐luciferase reporter, and RNA pull‐down assays were utilized to assay the targeted interactions among the variables. Results The TCF7 and SEMA3A levels were elevated in serum from patients with diabetic nephropathy. TCF7 silencing or SEMA3A depletion ameliorated high glucose (HG)‐induced podocyte injury. Moreover, TCF7 silencing protected against HG‐induced podocyte injury by down‐regulating SEMA3A. TCF7 targeted miR‐16‐5p, and miR‐16‐5p targeted SEMA3A. Furthermore, TCF7 affected the expression of SEMA3A by competing specifically for shared miR‐16‐5p. Conclusions These findings suggested that TCF7 silencing attenuated high glucose‐induced podocyte damage partially through the miR‐16‐5p/SEMA3A regulation cascade.

Our findings suggest that auricular acupuncture may be a viable alternative for the treatment of flat warts. Larger randomized studies are needed to fully evaluate auricular acupuncture against more conventional treatments, and these are planned.

A visible-light photocatalytic generation of iminoxyl radicals has been accomplished under oxidant-free conditions. This approach offers a mild, atom-economical, and redox-neutral synthesis of 5-methylisoxazolines through radical hydroxygenation of β,γ-unsaturated oximes in the absence of an additional hydrogen source.