To explore the application of artificial intelligence interaction technology in preschool education practice courses, preschool education, 5G wireless network, artificial intelligence interaction technology, and other relevant theories are integrated, questionnaire survey is adopted as the research method, and six-year-old kindergarten students in a city of China are taken as the research objects. Adaptive optimization suggestions are put forward from the preschool children practice curriculum learning situation, cooperation and exchange situation, learning effect, and learning attitude research. The results show that in terms of learning of practical courses, students’ cognition of learning has changed after the implementation of wisdom teaching, but the average value of some P values < 0.05 is low in the real classroom adaptation stage. In the exchange and cooperation of practical courses, it is found that wisdom teaching has the greatest influence on helping other students solve problems, with a specific P of 0.009. In terms of learning effect and learning attitude, the effect is significant after the introduction of artificial intelligence technology, with an average of 3.56 before the introduction and 4.01 after the introduction. It means that to a certain extent, preschool children can realize the role and significance of wisdom classroom teaching, which plays a certain role in teaching practice. Therefore, research on the application of artificial intelligence interaction technology in preschool education practice courses under 5G wireless network environment provides a new theoretical basis and optimization direction for preschool education in the future.

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