Ischemic stroke continues to be a serious public health problem worldwide, we compared the amino acid profiles of convalescent patients with ischemic stroke with healthy subjects by High Performance Liquid Chromatography (HPLC), 48 convalescent patients with ischemic stroke and 48 healthy controls were recruited, finding that the levels of Phosphoserine(P-Ser), Glutamic Acid(Glu), Isoleucine(Ile), Leucine(Leu), Phenylalanine(Phe), Arginine(Arg), Proline(Pro) in stroke patients increased significantly, and the levels of Taurine(Tau), Cystine(Cys), Methionine(Met), b-Alanine(b-Ala), Histidine(His) lowered in patients compared to controls (P<0.05). Furthermore, it was particularly important to find indicators that could judge the prognosis of patients, the Tau/Glu level could be used as a potential prognosis marker and considering convalescent stroke patients have a good prognosis when the ratio > 0.79 in our study.