The key to achieving maximum yields from microcarrier cultures is by using the optimization factors as replenishment of vero cell culture media during growth, the inoculation density on the proportion of microcarriers bearing cells, stirring speed on the growth of vero cell on cytodex 3, culture volume and headspace on cell yield from closed microcarrier system, various culture media, various types of the serum, control of PH, microcarrier concentration and modified initial culture procedure. The result reveled that the higher cell yield obtained after replenishment of culture media every three day, the optimum concentration of microcarrier is to be 3mg cytodex/ml, the optimum inoculation density 15x10 6 cells/mg cytodex microcarrier is required, the higher cell yield obtained by using (medium 199) containing high amount of amino acid at low density of the cell higher than Dulbecco's modification of Eagles's medium (DME) in higher density of the cell, fetal calf serum gave higher cell yield in the first three days in the culture, 60 rpm was the optimum stirring speed to get higher cell yield, addition of 10-25 mμ Hepes helped in maintaining the PH around 7.3-7.4 this gave higher cell yield, reduced the head space not more than half full of the culture give a higher result and the modified in initial culture procedure during the growth culture give higher results. The obtained result maximizing the yield of the vero cell production on acytodex-3 microcarrier culture system.

Peste des petits ruminants (PPR) is considered one of the most dangerous viral diseases of small ruminants in Africa and Asian countries. The disease control is based on vaccination of susceptible animals with an attenuated PPR virus strain (Nigeria 75/1) propagated in monolayers of Vero cells using stationery and roller flasks. This study for constructing alternative method for production of PPR vaccinal strain through propagation of PPR virus (75/l) on Vero cell culture supported on cytodex-three microcarrier beads using spinner stirring flasks and compare the recorded results with that obtained from stationary flasks (175cm 2) and Roller Bottles. All cultures were propagated under the same conditions (media, pH, time of incubation) and infected with the same multiplicity of infection by PPR vaccinal virus. Samples were obtained daily for successive five days from all cultures. The PPR virus titers were 12 log10 TCID50, 5 log10 TCID50 and 6 log10 TCID50 on microcarriers culture, stationary system and roller system respectively after six days post infection. These results provide further insights to apply microcarriers cell culture technology in production of PPR vaccine.